We provide world-class transformation and gene editing services for a wide range of wheat varieties, including elite and research-preferred cultivars.

Our facility utilizes Agrobacterium-mediated techniques and immature embryos as the donor material for transformation, ensuring high-efficiency results across various wheat genotypes.

We specialize in Agrobacterium-mediated transformation and gene editing for a wide range of wheat cultivars, including elite and research-preferred varieties.

Tested Varieties:

• Fielder
• Cadenza
• Kronos
• Paragon
• Reedling-Borlaug 100
• Skyfall
• Svevo
• Selected winter wheat varieties

We are continually developing protocols for other genotypes, including more elite varieties.

Our Services

Transformation and Gene Editing Services

Standard Variety: Fielder

• T0 transgenic plants with copy number analysis to support your research.
• Single/multiplex gene knockout services
• Timeline: The whole transformation process takes around 5 months after the construct is ready and introduced into the plant material.
• Custom Requests: If you need other wheat varieties, please provide 4–5 months lead time for growing the donor material.

Cloning Services

Our platform provides efficient cloning services for a variety of applications, including:

• CRISPR/Cas (including gRNA design)
• Gene of interest / Complementation studies
• Overexpression
• RNA interference (RNAi)

All cloning is into our preferred backbones designed for wheat Agrobacterium-transformation, ensuring the highest efficiency and compatibility.

Contact: Mark Smedley, Wheat Transformation Construct Lead, for advice and cloning services.

Our Expertise Includes

• Development of advanced gene editing tools for targeted genome mutations including base editing and prime editing.
• Continue development of bespoke protocols for wheat genotypes, including more elite varieties and landraces.

Our team has extensive expertise in cutting edge gene editing technologies and are happy to discuss potential research collaborations.

Contact: Dr. Sadiye Hayta, Wheat Transformation Lead, to discuss your project requirements and explore potential collaborations.

Protocol Publications

• Hayta S, Smedley MA, Clarke M, Forner M, Harwood W (2021). An Efficient Agrobacterium-Mediated Transformation Protocol for Hexaploid and Tetraploid Wheat, Current Protocols (1 (3)), Publisher’s version: 2691-1299
• Smedley M, Hayta S, Clark M, Harwood W (2021). CRISPR-Cas9 Based Genome Editing in Wheat. Current Protocols (Current Protocols Volume 1, Issue 3 e65). Publisher’s version: 2379-8068
• Debernardi J, Tricoli D, Ercoli M, Hayta S, Ronald P, Palatnik J, Dubcovsky J (2020). A GRFGIF chimeric protein improves the regeneration efficiency of transgenic plants. Nature Biotechnology (online). Publisher’s version: ISSN 1546-1696 (online)