Nuclear Magnetic Resonance encompasses several powerful structural and analytical methodologies which are capable of providing atomic level information about molecules.
For example, Nuclear Magnetic Resonance can determine the numbers of particular atoms within a molecule, their chemical environments and how they are interconnected and spatially arranged based on their magnetic properties.
At the John Innes Centre, there are two solution-state instruments which application depends on the amount of available sample and specific analytical task. Both instruments are highly automated being configured with autosamplers and supported by advanced software packages, providing comprehensive characterisation of the systems under investigation.
- Structural analysis of new compounds
- Metabolite identification
- Monitoring of enzymatic reactions
- Understanding enzyme specificity and mode of action
The enhanced sensitivity of the 600 MHz spectrometer due to cryoprobe makes it advantageous for collecting data for NMR metabolomics profiling while triple resonance characteristics of this probe also allow conducing biomolecular NMR experiments.
While the NMR facility is is primarily used for academic research across Norwich Research Park, the team has sufficient capacity to accommodate external work, including as a package with metabolomics mass spectrometry or high-resolution mass spectrometry.
Prices vary according to the type of experiments and sample quantities involved, contact the team for a bespoke quote for your project.
- 600 MHz Bruker Avance Neo spectrometer equipped with a helium-cooled triple resonance cryoprobe
- 400 MHz Bruker Avance III spectrometer equipped with a broadband probe