Technology and Research Platforms

Crop Transformation (Brassica)

The Crop Transformation Platform offers Agrobacterium-mediated transformation of crop and model species, construct design, construct assembly and genome editing. This platform provides for the John Innes Centre researchers and broader scientific community.

The facility operates on a not-for-profit, cost recovery basis, supporting researchers globally. Our team can collaborate with you to secure funding through internal SSA awards (JIC staff only) or assist with cost breakdowns and advice for grant applications.

What we offer

  • Brassica napus
  • Brassica oleracea
  • Tomato
  • Potato
  • Arabidopsis thaliana
  • Medicago truncatula
  • Construct design
  • Genome editing – single/multiplex knockout and more specified outcomes
  • T-DNA copy number assay of transgenic lines
  • Genotyping of transgenic/edited lines (in association with Molecular Genetics platform)
  • Delivery of primary transgenics
  • Delivery of T1 seed

Get in touch

Please get in touch to discuss your specific needs. We are here to assist with any questions or specific requests related to your transformation and gene editing projects.

For more information about Brassica transformation, contact Tom Lawrenson (tom.lawrenson@jic.ac.uk)

We are active in developing capability in other species and technologies:

  • Pea transformation
  • Sugar beet transformation
  • HDR based, precise, predefined editing outcomes
  • Base editing

Explore our collection of training videos on the John Innes Centre YouTube channel, where you can find transformation tutorials for some of the crops we work with.