Technology and Research Platforms

Biophysical Analysis

The Biophysical Analysis platform offers four techniques; Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Mass Photometry (MP) and Circular Dichroism Spectroscopy (CD).

SPR and ITC are complimentary techniques that can be used to study the binding of biomolecules. They can be used to identity interactions and obtain data on the affinity, kinetics, stoichiometry and thermodynamics of the binding interaction.

Mass Photometry enables the accurate mass determination of single molecules in solution and can be used to determine the oligomeric state of proteins and characterise biomolecules. The instrument can study samples with a wide mass range 40kDa-5MDa and is quick, sensitive and uses minimal sample amounts

CD spectroscopy is used to investigate the structural properties of biological molecules in solution. It is regularly used to check the quality of protein purification batches or to compare the structures of mutant and wild type proteins to ensure the mutation hasn’t affected the folding of the protein.

The Biophysical Analysis team can help design experiments and can advise the best techniques and experiment design prior to work being carried out. They can take on both academic and commercial projects.

The team offer training courses in all techniques and individual bespoke training to enable customers to be able to run the experiments themselves. The option is also there for the team to do all the work and deliver the results in a report.

To arrange a discussion email Dr Abbas Maqbool. It is best to do this prior to preparing any samples so the best advice can be given.

All artwork by Julia Mundy.