Proteomics

The John Innes Centre Proteomics Facility provides proteomic services using mass spectrometry to the institutions across Norwich Research Park and externally on request.

Proteomic technologies, and supporting expertise, enable us to identify the entire protein complement (proteome) of a sample, whether from a cell or whole organism.

Comparing a partial amino acid sequence for a protein with an organism's genome sequence immediately enables recognition of both the corresponding gene and the protein's complete amino acid sequence.

Proteomics links the static information contained in the organism's DNA (its genotype) to the dynamic physiology of the whole-cell and/or whole-organism (its phenotype).

By analyzing the abundance of proteins under different physiological conditions, or in different mutants, it is possible to identify groups of proteins that are important under those particular physiological conditions or are affected by that specific mutation. The amino acid sequence of the proteins identifies the genes involved.

Ongoing advances in automated sample preparation, mass spectrometry and bio-informatics are making it possible to quickly and accurately relate proteins to genes.

The facility is equipped with four state-of-the-art mass spectrometers covering a wide range of applications and a number of state-of-the-art programmes for data processing, protein identification by database search, and quantification, so that optimal applications are available for different needs of sample analysis.

This allows for a broad range of methods including analysis of individual proteins (e.g. intact mass, sequencing, identification) as well as qualitative and quantitative analysis of complex protein samples (e.g. pull-downs, expression studies, modifications).

The Proteomics equipment and services at the John Innes Centre include;

  • Orbitrap-Fusion (Thermo) with nanoLC
  • Synapt G2Si (Waters) with UPLC, nanoLC, Maldi, DESI
  • Autoflex Speed (Bruker)
  • Xevo TQS Triple Quad (Waters) with nanoLC
  • Analysis of intact proteins: intact mass by Maldi-Tof or LCMS-ESI , N-terminal sequencing (Maldi ISD)
  • DESI imaging on Synapt G2Si
  • Identification/ characterisation of individual proteins by Maldi PMF or nanoLC Oribtrap
  • Analysis of complex protein samples (total cell extracts, CoIP’s etc) using nanoLC Orbitrap or nanoLC MSE (Synapt G2Si)
  • Quantitative analysis of proteomics samples by label free quantification or using isotope labelling (e.g. iTRAQ, TMT, 15N)
  • Ion mobility spectroscopy on Synapt G2Si
  • Maldi imaging on Synapt G2Si and on Autoflex speed

"The John Innes Centre Proteomics Facility provides services in the area of proteomics using mass spectrometry. The facility is equipped with four state-of-the-art mass spectrometers covering a wide range of applications. This allows for a broad range of methods including analysis of individual proteins (e.g. intact mass, sequencing, identification), the qualitative and quantitative analysis of complex protein samples (e.g. pull-downs, expression studies, modifications, isotope labelling), as well as mass spectrometry imaging and ion mobility"

- Gerhard Saalbach, Proteomics Senior Scientist

JIC Cookie Policy. We use cookies on this site to enhance your user experience. By clicking any link on this page you are giving your consent for us to set cookies. You can find out more about the cookies by clicking here.

Accept cookies