Biomolecular Analysis

Nuclear Magnetic Resonance

Nuclear Magnetic Resonance encompasses several powerful structural and analytical methodologies which are capable of providing atomic level information about molecules.

For example, Nuclear Magnetic Resonance can determine the numbers of particular atoms within a molecule, their chemical environments and how they are interconnected and spatially arranged based on their magnetic properties.

Within the facility there are two solution-state instruments which application depends on the amount of available sample and specific analytical task.

Both instruments are highly automated being configured with autosamplers and supported by advanced software packages.

The spectrometers software is set up to run a wide variety of Nuclear Magnetic Resonance experiments designed for comprehensive characterisation of the systems under investigation.

New high-field Nuclear Magnetic Resonance instrument has been commissioned by Bruker. The 600 MHz Avance Neo spectrometer with cryoprobe will enhance molecular characterisation which is central for our Molecules from Nature strategic programme.

“We support the science at the John Innes Centre with Nuclear Magnetic Resonance Spectroscopy for structural analysis of new compounds, metabolite identification, monitoring enzymatic reactions and understanding enzyme specificity and mode of action”

– Sergey Nepogodiev, Nuclear Magnetic Resonance Spectroscopy


400 MHz Bruker Avance III spectrometer equipped with a broadband probe

  • Typical usage of 400 MHz spectrometer is recording routine 1D 1H and 13C NMR spectra as well as conducting 2D analyses of small and medium sized molecules
  • A 60-positions sample changer provides medium throughput analysis
  • The multinuclear probe of 400 MHz spectrometer is tunable for measuring NMR of all NMR active nuclei, e.g. 31P and 19F NMR
  • Experiments can be conducted in a range of -150 to +150 °C

600 MHz Bruker Avance Neo spectrometer equipped with a helium-cooled cryoprobe

  • 600 MHz spectrometer is dedicated to more sophisticated analyses of natural products as well as products of chemical and biochemical reactions when high sensitivity is paramount due to low sample concentrations
  • Triple (TCI) resonance cryoprobe also allows to perform double and triple resonance experiments developed for structure determination of biological macromolecules
  • Variable temperature capability of 600 MHz instrument is in the range of -20 to +80 °C