Prof Saskia Hogenhout
Cell and Developmental Biology
Research in the Hogenhout lab centres on Molecular-Plant-Microbe-Insect Interactions (MPMII).
Our first goal is to better understand the molecular basis of plant interactions with insect herbivores, particularly aphids, leafhoppers and whiteflies (hemipterans). These insects produce virulence proteins (effectors) in their saliva that modulate plant responses and aid insect colonization. We have identified insect effectors and are in the process of analysing their functions by finding plant targets and knocking down corresponding genes in the insects by plant-mediated RNA interference (RNAi).
Hemipteran insects are efficient transmitters of a diverse range of plant pathogens, predominantly viruses and bacteria, and hence may be viewed as the ‘mosquitoes of plants’. Therefore, our second goal is to understand plant-insect-pathogen interactions. Leafhoppers transmit phytoplasmas, which are bacterial plant pathogens related to mycoplasmas. Phytoplasmas induce dramatic changes in plant development, including proliferation of stems (witch’s brooms) and the reversion of flowers into leaf-like organs (phyllody). We have identified phytoplasma effectors that induce these phenotypes by interacting and degrading conserved proteins, including TCPs and MADS-box transcription factors. Plants also become very susceptible and attractive to insect vectors on which the phytoplasmas depend for transmission to other plants. Thus, phytoplasmas appear to completely hijack their plant hosts changing them into ‘zombies’ destined to maximise pathogen survival.
By definition, studies on MPMII are interdisciplinary and require expertise with at least three unrelated organisms. We work in close collaboration with Dr Ian Bedford and the Entomology facility at the John Innes Centre.
The small phytoplasma virulence effector SAP11 contains distinct domains required for nuclear targeting and CIN-TCP binding and destabilization.
New Phytologist 202 (3) p838-48
Publisher’s version: 10.1111/nph.12721
The Leucine-Rich Repeat Receptor-Like Kinase BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 and the Cytochrome P450 PHYTOALEXIN DEFICIENT3 Contribute to Innate Immunity to Aphids in Arabidopsis.
Plant Physiology 164 (4) p2207-19
Publisher’s version: 10.1104/pp.114.235598
Phytoplasma Effector SAP54 Hijacks Plant Reproduction by Degrading MADS-box Proteins and Promotes Insect Colonization in a RAD23-Dependent Manner.
PLoS Biology 12 (4) pe1001835
Publisher’s version: 10.1371/journal.pbio.1001835
Methods in Molecular Biology 1127 p125-36
Publisher’s version: 10.1007/978-1-62703-986-4_10
Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids.
Methods in Molecular Biology 1127 p137-43
Publisher’s version: 10.1007/978-1-62703-986-4_11
- Javier Galdon Armero Postgraduate Student
- Friederike Bernsdorff Postdoctoral Scientist
- Christine Wilson Postgraduate Student
- Dr Vera Thole Postdoctoral Scientist
- Pascal Pecher Postdoctoral Scientist
- Zigmunds Orlovskis Postgraduate Student
- Dr Sam Mugford Research Assistant
- Emma Knowles Postgraduate Student
- Claire Drurey Postgraduate Student
- Dr Yazhou Chen Postdoctoral Scientist
- Dr Ian Bedford Senior Scientist
- Thomas Vincent Postgraduate Student
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