Prof Mark Buttner
Head of Department
Research in our lab focusses on the cell biological processes underpinning Streptomyces sporulation, the master regulators that control these processes, and the regulatory networks that link the two.
Our approach exploits a broad range of disciplines including cell biology, modelling, global ‘omics’ methods, genetics and biochemistry, right through to X-ray crystallography. As part of our work, we have recently pioneered the development of Streptomyces venezuelae as a new model system for the genus. Unlike most Streptomyces species, S. venezuelae sporulates in liquid culture, giving us a unique opportunity to follow the movement of multiple developmental proteins in time and space during differentiation using time-lapse imaging (movies). Sporulation in liquid culture also allows us to optimally apply global approaches like microarray transcriptional profiling and ChIP-seq to the analysis of cellular differentiation.
In addition to our developmental research, we also investigate the molecular mechanisms that mediate oxidative and cell envelope stress responses in Streptomyces, and those involved in the control of antibiotic resistance, focussing on key components of each signal transduction pathway.
Genome-wide ChIP-seq analysis shows that WhiB is a transcription factor that co-controls its regulon with WhiA to initiate developmental cell division in Streptomyces
mBio 7 pe00523-16
Publisher’s version: 10.1128/mBio.00523-16
Journal of Visualized Experiments 108 pe53863
Publisher’s version: 10.3791/53863
Nature Reviews Microbiology 13 p749-60
Publisher’s version: 10.1038/nrmicro3546
SimC7 is a novel NAD(P)H-dependent ketoreductase essential for the antibiotic activity of the DNA gyrase inhibitor simocyclinone.
Journal of Molecular Biology 427 p2192-2204
Publisher’s version: 10.1016/j.jmb.2015.03.019
Discovery of a family of γ-aminobutyrate ureas via rational derepression of a silent bacterial gene cluster
Chemical Science 5 p86-89
Publisher’s version: 10.1039/c3sc52536h
- Dr Matt Bush Research Assistant
- Dr Morgan Feeney Postdoctoral Scientist
- Martin Schafer Postgraduate Student
- Dr Susan Schlimpert Research Fellow
- Kelley Gallagher Postdoctoral Scientist
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