Prof Mark Buttner
Head of Department
Research in our lab focusses on the cell biological processes underpinning Streptomyces sporulation, the master regulators that control these processes, and the regulatory networks that link the two.
Our approach exploits a broad range of disciplines including cell biology, modelling, global ‘omics’ methods, genetics and biochemistry, right through to X-ray crystallography. As part of our work, we have recently pioneered the development of Streptomyces venezuelae as a new model system for the genus. Unlike most Streptomyces species, S. venezuelae sporulates in liquid culture, giving us a unique opportunity to follow the movement of multiple developmental proteins in time and space during differentiation using time-lapse imaging (movies). Sporulation in liquid culture also allows us to optimally apply global approaches like microarray transcriptional profiling and ChIP-seq to the analysis of cellular differentiation.
In addition to our developmental research, we also investigate the molecular mechanisms that mediate oxidative and cell envelope stress responses in Streptomyces, and those involved in the control of antibiotic resistance, focussing on key components of each signal transduction pathway.
Tetrameric c-di-GMP Mediates Effective Transcription Factor Dimerization to Control Streptomyces Development.
Cell 158 (5) p1136-47
Publisher’s version: 10.1016/j.cell.2014.07.022
PLoS Genetics 10 (8) pe1004554
Publisher’s version: 10.1371/journal.pgen.1004554
Genes required for aerial growth, cell division and chromosome segregation are targets of WhiA before sporulation in Streptomyces vene.zuelae
Publisher’s version: 10.1128/mBio.00684-13
The Ser/Thr protein kinase AfsK regulates polar growth and hyphal branching in the filamentous bacteria Streptomyces.
Proceedings of the National Academy of Sciences of the United States of America (35)
Publisher’s version: 10.1073/pnas.1207409109
Expression of the chaplin and rodlin hydrophobic sheath proteins in Streptomyces venezuelae is controlled by σBldN and a cognate anti-sigma factor, RsbN.
Molecular Microbiology 84 (6) p1033-1049
Publisher’s version: 10.1111/j.1365-2958.2012.08070.x
SimC7 is a novel NAD(P)H-dependent ketoreductase essential for the antibiotic activity of the DNA gyrase inhibitor simocyclinone.
Journal of Molecular Biology 427 p2192-2204
Publisher’s version: 10.1016/j.jmb.2015.03.019
Discovery of a family of γ-aminobutyrate ureas via rational derepression of a silent bacterial gene cluster
Chemical Science 5 p86-89
Publisher’s version: 10.1039/c3sc52536h
A sporulation-specific, sigF-dependent protein, SspA, affects septum positioning in Streptomyces coelicolor.
Molecular Microbiology 91 (2) p363-80
Publisher’s version: 10.1111/mmi.12466
Determination of Phosphorylation Sites in the DivIVA Cytoskeletal Protein of Streptomyces coelicolor by Targeted LC-MS/MS.
Journal of Proteome Research 12 (9) p4187-92
Publisher’s version: 10.1021/pr400524d
- Dr Matt Bush Research Assistant
- Dr Morgan Feeney Postdoctoral Scientist
- Martin Schafer Postgraduate Student
- Dr Susan Schlimpert Research Fellow
- Kelley Gallagher Postdoctoral Scientist
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