Prof Mark Buttner
Head of Department
Mark works on the control of development in the antibiotic-producing bacteria Streptomyces.
His lab has established S. venezuelae as a new model species for the genus because it sporulates in liquid culture. This greatly facilitates the application of global ‘omics’ approaches to the study of development, and allows movies to be made of the complete spore-to-spore lifecycle.
Mark also uses Streptomyces superhost strains to express antibiotic gene clusters heterologously.
- Developmental research into sporulation in Streptomyces
- Sporulation in liquid culture
- Streptomyces superhosts for heterologous expression of antibiotic gene clusters
Research in the lab focusses on the cell biological processes underpinning Streptomyces sporulation, the master regulators that control these processes, and the regulatory networks that link the two.
Their approach exploits a broad range of disciplines including cell biology, modelling, global ‘omics’ methods, genetics and biochemistry, right through to X-ray crystallography.
As part of thier work, we have recently pioneered the development of Streptomyces venezuelae as a new model system for the genus. Unlike most Streptomyces species, S. venezuelae sporulates in liquid culture, giving us a unique opportunity to follow the movement of multiple developmental proteins in time and space during differentiation using time-lapse imaging (movies). Sporulation in liquid culture also allows us to optimally apply global approaches like microarray transcriptional profiling and ChIP-seq to the analysis of cellular differentiation.
In addition to thier developmental research, we also investigate the molecular mechanisms that mediate oxidative and cell envelope stress responses in Streptomyces, and those involved in the control of antibiotic resistance, focussing on key components of each signal transduction pathway.
Proceedings of the National Academy of Sciences of the United States of America 114 pE6176-E6183
Publisher’s version: 10.1073/pnas.1704612114
Molecular Microbiology 104 p700-711
Publisher’s version: 10.1111/mmi.13663
Journal of Bacteriology 199 pe00070-17
Publisher’s version: 10.1128/JB.00070-17
Translational Control of the SigR-Directed Oxidative Stress Response in Streptomyces via IF3-Mediated Repression of a Noncanonical GTC Start Codon.
mBio 8 pe00815-17
Publisher’s version: 10.1128/mBio.00815-17
The Streptomyces master regulator BldD binds c-di-GMP sequentially to create a functional BldD2-(c-di-GMP)4 complex.
Nucleic acids research 45 p6923-6933
Publisher’s version: 10.1093/nar/gkx287
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