Saleha leads the technology platform service RevGenUK which offers TILLING (Targeting Induced Local Lesions in Genomes) on populations of mutagenized model and crop species.
TILLING is a ‘reverse genetics’ process used to identify mutants in a specific gene of interest.
The TILLING populations available are:
- Brassica napus (Cabriolet)
- Brassica oleracea (DH1012)
- Capsella rubella (SAS 52)
- Lotus japonicus (Gifu and MG20)
- Medicago truncatula (A17)
- Avena strigosa (S75)
The Brassica rapa (R-o-18) population is an off the shelf service made up of over 1,000 re-sequenced lines available through the RevGenUK website.
Miller C., Wells R., McKenzie N., Trick M., Ball J., Fatihi A., Dubreucq B., Chardot T., Lepiniec L., Bevan M. W. (2019)Variation in Expression of the HECT E3 Ligase UPL3 Modulates LEC2 Levels, Seed Size, and Crop Yields in Brassica napus.Plant Cell (31)Publisher's version: 1040-4651
Stephenson P., Stacey N., Bruser M., Pullen N., Ilyas M., O'Neill C., Wells R., Ostergaard L. (2019)The power of model-to-crop translation illustrated by reducing seed loss from pod shatter in oilseed rape.Plant reproductionPublisher's version: 2194-7953
Bakht S,Bakht S,Poza-Viejo L,Piñeiro M,Jarillo JA,Crevillén P (2019)High ambient temperature leads to reduced FT expression and delayed flowering in Brassica rapa via a mechanism associated with H2A.Z dynamics.The Plant journal : for cell and molecular biologyPublisher's version: 0960-7412