Reverse Genetics

The Reverse Gentics equipment and services at the John Innes Centre include;

  • TILLING (service)
  • ABI3730XL used for SNP detection and sequencing
  • Fragment Analyser (AATI) used for SNP detection
  • Biomek NxP Liquid Handler (Beckman Coulter) for population development

"We offer a bespoke TILLING service on our population of mutagenized model and crop species. You let us know what gene you would like to investigate and we will pick the optimum region to TILL, design primers, carry out TILLING and return a list of mutations. You can pick the mutations you are interested in and request seeds of the lines bearing those mutations"

- Saleha Bakht, Crop Genetics

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