John Innes Centre site search
Alison Smith
Department of Metabolic Biology
Alison Smith

Publications since 1998:

Tomlinson K, Craig J, Smith AM (1998) Major differences in isoform composition of starch synthase between leaves and embryos of pea (Pisum sativum L.) Planta 204, 86-92.

Craig J, Lloyd JR, Tomlinson K, Barber L, Edwards A, Wang TL, Martin C, Hedley CL, Smith AM (1998) Mutations in the gene encoding starch synthase II profoundly alter amylopectin structure in pea embryos. Plant Cell 10, 413-426.     

Purcell P, Smith AM, Halford NG (1998) Antisense expression of a sucrose nonfermenting-1-related protein kinase sequence in potato results in decreased expression of sucrose synthase in tubers and loss of sucrose-inducibility of sucrose synthase transcripts in leaves. Plant J. 14, 195-202.                                                                

Zhu ZP, Hylton CM, Rössner U, Smith AM (1998) Characterisation of starch-debranching enzymes in pea (Pisum sativum L.) embryos. Plant Physiol. 118, 581-510.   

Zeeman SC, Northrop F, Smith AM, ap Rees T (1998) A starch-accumulating mutant of Arabidopsis thaliana deficient in a chloroplastic starch-hydrolysing enzyme. Plant J. 15, 357-365.                                                                                         

Zeeman SC, Umemoto T, Lue WL, Au-Yeung P, Martin C, Smith AM, Chen J (1998) A mutant of Arabidopsis thaliana lacking a chloroplastic isoamylase accumulates both starch and phytoglycogen. Plant Cell 10, 1699-1711.                

Edwards A, Fulton D, Hylton CM, Jobling S.A, Gidley M, Rössner U, Martin C, Smith AM (1999) A combined reduction in activity of starch synthases II and III of potato has novel effects on the starch of tubers. Plant J. 17, 251-261.                          

Craig J, Barratt P, Tatge H, Déjardin A, Handley L, Gardner CD, Barber L, Wang T, Hedley C, Martin C, Smith AM (1999) Mutations at the rug4 locus alter the carbon and nitrogen metabolism of pea plants through an effect on sucrose synthase. Plant J. 17, 353-362.

Tatge H, Marshall J, Martin C, Edwards EA, Smith AM (1999) Evidence that amylose synthesis occurs within the matrix of starch granules. Plant Cell Env. 22, 543-550.

Denyer K, Waite D, Motawia S, Lindberg-Møller B, Smith AM (1999) Granule-bound starch synthase I in isolated starch granules elongates malto-oligosaccharides processively. Biochem. J. 340, 183-191.                     

Edwards A, Borthakur A, Bornemann S, Venail J, Denyer K, Waite D, Fulton D, Smith AM, Martin C (1999) Specificity of starch synthase isoforms from potato. Eur. J. Biochem. 266, 724-726.    

Clarke BR, Denyer K, Jenner CF, Smith AM (1999) The relationship between the rate of starch synthesis, the adenosine 5’-diphosphoglucose concentration and the amylose content of starch in developing pea embryos. Planta 209, 324-329.

Denyer K, Waite D, Edwards A, Martin C, Smith AM (1999) Interaction with amylopectin influences the ability of granule-bound starch synthase I to elongate malto-oligosaccharides processively. Biochem. J. 342, 647-653.  

Beckles DM, Smith AM, ap Rees T (2001) A cytosolic ADPglucose pyrophosphorylase is a feature of graminaceous endosperms, but not of other starch-storing organs. Plant Physiol. 125, 818-827.

Beckles DM, Smith AM (2001) ADPglucose pyrophosphorylase is located in the plastid in developing tomato fruit. Plant Physiol. 126, 261-266.

Critchley JH, Zeeman SC, Takaha T, Smith AM, Smith SM (2001) A critical role for disproportionating enzyme in starch breakdown is revealed by a knock-out mutation in Arabidopsis. Plant J. 26, 89-100.

Barratt DHP, Barber L, Kruger NJ, Smith AM, Wang TL, Martin C (2001) Multiple, distinct isoforms of sucrose synthase in pea. Plant Physiol. 127, 655-664.

Smith AM (2001) The biosynthesis of starch granules. Biomacromolecules 2, 335-341.

Motawia MS, Olsen CE, Denyer K, Smith AM, Lindberg-Møller B. (2001) Synthesis of 4’-O-acetyl-maltose and D-galactopyranosyl-D-glucopyranose for biochemical studies of amylose biosynthesis. Carbohydr. Res. 330, 309-318.

Yu TS, Kofler H, Häusler RE, Hille D, Flügge UI, Zeeman SC, Smith AM, Kossmann J, Lloyd J, Ritte G, Steup M, Lue WL, Chen J, Weber A (2001) The Arabidopsis sex1 mutant is defective in the R1 protein, a general regulator of starch degradation in plants, and not in the chloroplast hexose transporter. Plant Cell 13, 1907-1918.

Fulton DC, Edwards A, Pilling E, Robinson HL, Fahy B, Seale R, Kato L, Donald AM, Geigenberger P, Martin C, Smith AM (2002) Role of granule-bound starch synthase in determination of amylopectin structure and starch granule morphology in potato. J. Biol. Chem. 277, 10834-10841.

Zeeman SC, Smith SM, Smith AM (2002) The priming of amylose synthesis in Arabidopsis leaves.  Plant Physiol. 128, 1069-1076.

Zeeman SC, Tiessen A, Pilling E, Kato L, Donald AM, Smith AM (2002) Starch synthesis in Arabidopsis. Granule synthesis, composition, and structure. Plant Physiol. 129, 516-529.

Edwards A, Vincken JP, Suurs LCJM, Visser RGF, Zeeman S, Smith A, Martin C (2002) Discrete forms of amylose are synthesized by isoforms of granule-bound starch synthase in pea. Plant Cell 14, 1767-1785.

Patron N, Smith AM, Fahy B, Hylton CM, Naldrett M, Rossnagel BG, Denyer K (2002) A mutation in the 5’ non-coding region of the barley GBSSI gene alters its temporal and spatial expression and reduces GBSSI activity and amylose content in the endosperm. Plant Physiol. 130, 190-198.

Hussain H, Mant A, Seale R, Zeeman S, Hinchliffe E, Edwards A, Hylton C, Bornemann S, Smith AM, Martin C, Bustos R (2003) Three isoforms of isoamylase contribute different catalytic properties for the debranching of potato glucans. Plant Cell 15, 133-149.

Pilling E, Smith AM (2003) Growth ring formation in the starch granules of potato tubers. Plant Physiol. 132, 365-371.

Smith AM, Zeeman S, Thorneycroft D, Smith SM (2003) Starch mobilization in leaves. J. Exp. Bot. 54, 577-583.

Smith AM, Zeeman SC, Niittylä T, Kofler H, Thorneycroft D, Smith SM (2003) Starch degradation in leaves. J. Appl. Glycosci. 50, 173-176.

Niittylä T, Messerli G, Trevisan M, Chen J, Smith AM, Zeeman SC (2004) A novel maltose transporter essential for starch degradation in leaves. Science 303, 87-89.

Chia T, Thorneycroft D, Chapple A, Messerli G, Chen J, Zeeman SC, Smith SM, Smith AM (2004) A cytosolic glucosyltransferase is required for conversion of starch to sucrose in Arabidopsis leaves at night. Plant J. 37, 853-863.

Bustos R, Fahy B, Hylton CM, Seale R, Nebane NM, Edwards A, Martin C, Smith AM (2004) Starch granule initiation is controlled by a heteromultimeric isoamylase in potato tubers. Proc. Natl Acad. Sci. USA 101, 2215-2220.

Zeeman SC, Thorneycroft D, Schupp N, Chapple A, Weck M, Dunstan H, Halidmann P, Bechtold N, Smith AM, Smith SM (2004) Plastidial α-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress. Plant Physiol. 135, 849-858.

Zeeman SC, Smith SM, Smith AM (2004) The breakdown of starch in leaves. New Phytol. 163, 247-261.

Smith SM, Fulton DC, Chia T, Thorneycroft D, Chapple A, Dunstan H, Hylton C, Zeeman SC, Smith AM (2004) Diurnal changes in the transcriptome encoding enzymes of starch metabolism provide evidence for both transcriptional and posttranscriptional regulation of starch metabolism in Arabidopsis leaves. Plant Physiol. 136, 2687-2699.

Yu TS, Zeeman SC, Thorneycroft D, Fulton DC, Dunstan H, Lue WL, Hegemann B, Tung SY, Umemoto T, Chapple A, Tsai DL, Wang SM, Smith AM, Chen J, Smith SM (2005) α-amylase is not required for breakdown of transitory starch in Arabidopsis leaves. J. Biol. Chem. 280, 9773-9779.

Smith AM, Zeeman SC, Smith SM (2005) Starch degradation. Annu. Rev. Plant Biol. 56, 73-97.

Fettke J, Chia T, Eckermann N, Smith AM, Steup M (2006) A transglucosidase necessary for starch degradation and maltose metabolism in leaves acts on cytosolic heteroglycans. Plant J. 46, 668-684.

Niittylä T, Comparot-Moss S, Lue WL, Messerli G, Trevisan M, Seymour MDG, Gatehouse JA, Villadsen D, Smith SM, Chen J, Zeeman SC, Smith AM (2006) Similar protein phosphatases control starch metabolism in plants and glycogen metabolism in animals. J. Biol. Chem. 281, 11815-11818.

Smith AM, Zeeman SC (2006) Quantification of starch in plant tissues. Nature Protocols 1, 1342-1345

Bieniawska Z, Barratt DHP, Garlick AP, Thole V, Kruger NJ, Martin C, Zrenner R, Smith AM (2007) Analysis of the sucrose synthase gene family in Arabidopsis. Plant J. 49, 810-828.

Zeeman SC, Smith SM, Smith AM (2007) The diurnal metabolism of leaf starch. Biochem. J. 401, 13-28

Smith, AM, Stitt M (2007) Coordination of carbon supply and plant growth. Plant Cell Env. 30, 1128-1149.

Ceballos H, Sáchez T, Morante N, Fregene M, Dufour D, Smith AM, Denyer K, Pérez JC, Calle F, Mestres C (2007) Discovery of a waxy starch mutant in cassava. J. Agric. Food Chem. 55, 7469-7476.

Ceballos H, Sánchez T, Tofiño AP, Rosero EA, Denyer K, Smith A, Dufour D, Morante N, Pérez JC, Fahy B, Fregene M (2008) Induction and identification of a small-granule, high-amylose mutant in cassava (Manihot esculenta Crantz). J. Agric. Food Chem. 56, 7215–7222

Fulton DC, Stettler M, Mettler T, Vaughan CK, Li J, Franscisco P, Gil M, Reinhold H, Eicke, S, MesserliG, Dorken G, Halliday K, Smith AM, Smith SM, Zeeman, S.C. (2008) β-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active β-amylases in Arabidopsis chloroplasts. Plant Cell 20, 1040-1058.

Smith AM (2008) Prospects for increasing starch and sucrose yields for bioethanol production. Plant J. 54, 546-558.

Morley-Smith ER, Pike MJ, Findlay K, Kockenberger W, Hill LM, Smith AM, Rawsthorne S (2008) The transport of sugars to developing embryos is not via the bulk endosperm in Brassica napus seeds. Plant Physiol. 147, 2121-2130.

Tsesmetzis N, Couchman M, Higgins J, Smith A, Doonan JH, Seifert GJ, Schmidt EE, Vastrik I, Birney E, Wu G, D’Eustachio P, Stein LD, Morris RJ, Bevan MW, Walsh SV (2008) Arabidopsis Reactome: A foundation knowledgebase for plant systems biology. Plant Cell 20, 1426-1436.

Kötting O, Santelia D, Edner C, Eicke S, Marthaler T, Gentry MS, Comparot-Moss S, Chen J, Smith AM, Steup M, Ritte G, Zeeman SC (2009) STARCH-EXCESS4 is a laforin-like phosphoglucan phosphatase required for starch degradation in Arabidopsis thaliana. Plant Cell, 21: 334-346.

Barratt DHP, Derbyshire P, Findlay K, Pike M, Wellner N, Lunn J, Feil R, Simpson C, Maule A, Smith AM (2009) Normal growth of Arabidopsis plants requires cytosolic invertase but not sucrose synthase. Proc. Natl Acad. Sci. USA, doi:10.1073/pnas.0900689106.

Andriotis VM, Kruger NJ, Pike MJ, Smith AM(2009) Plastidial glycolysis in developing Arabidopsis embryos. New Phytol. 185: 649-662.

Andriotis, VME, Pike M, Kular B, Rawsthorne S, Smith AM (2010) Starch turnover in developing oilseed embryos. New Phytol. doi: 10.1111/j.1469-8137.2010.03311.x

Comparot-Moss S, Kötting O, Stettler M, Edner C, Graf A, Weise SE, Streb S, Lue WL, MacLean D, Mahlow S, Ritte G, Steup M, Chen J, Zeeman SC, Smith AM (2010) A putative phosphatase, LSF1, is required for normal starch turnover in Arabidopsis leaves. Plant Physiol. 152: 685-697.

Graf A, Schlereth A, Stitt M, Smith AM (2010) Circadian control of carbohydrate availability for growth in Arabidopsis plants at night. Proc. Natl. Acad. Sci. USA. 107: 9458-9463.

Andriotis VM, Pike M, Bunnewell S, Hills MJ, Smith AM (2010) The plastidial glucose-6-phosphate/phosphate antiporter GPT1 is essential for morphogenesis in Arabidopsis embryos. Plant J. 64,128-39.  doi:10.1111/j.1365-313X.2010.04313.x.

Zeeman SC, Kossmann J, Smith AM (2010) Starch: its metabolism, evolution, and biotechnological modification in plants. Annu. Rev. Plant Biol. 61, 209-34. doi:10.1146/annurev-arplant-042809-112301

Vriet C, Welham T, Brachmann A, Pike M, Pike J, Perry J, Parniske M, Sato S, Tabata S, Smith AM, Wang TL (2010) A suite of Lotus japonicus starch mutants reveals both conserved and novel features of starch metabolism. Plant Physiol. 154, 643-655.

Stanley D, Rejzek M, Naested H, Smedley M, Otero S, Fahy B, Thorpe F, Nash RJ, Harwood W, Svensson B, Denyer K, Field RA, Smith AM (2011) The role of alpha-glucosidase in germinating barley grains.  Plant Physiol.155, 932-943. doi: 10.1104/pp.110.168328.

Rejzek M, Stevenson CE, Southard AM, Stanley D, Denyer K, Smith AM, Naldrett MJ, Lawson DM, Field RA (2011) Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley beta-amylase. Mol. BioSyst. 7(3), 718-730. doi: 10.1039/c0mb00204f.

Graf A, Smith AM (2011) Starch and the clock: the dark side of plant productivity. Trends Plant Sci.16, 169-75. doi: 10.1016/j.tplants.2010.12.003.

Yandeau-Nelson MD, Laurens L, Shi Z, Xia H, Smith AM, Guiltinan MJ (2011) Starch-branching enzyme IIa is required for proper diurnal cycling of starch in leaves of maize. Plant Physiol. 156, 479-490. doi: 10.1104/pp.111.174094.

Barratt DHP, Kölling K, Graf A, Pike M, Calder G, Findlay K, Zeeman SC, Smith AM (2011) Callose synthase GSL7 is necessary for normal phloem transport and inflorescence growth in Arabidopsis. Plant Physiol.155, 328-41. doi: 10.1104/pp.110.166330.

Howard TP, Fahy B, Craggs A, Mumford R, Leigh F, Howell P, Greenland A, Smith AM (2012) Barley mutants with low rates of endosperm starch synthesis have low grain dormancy and high susceptibility to preharvest sprouting. New Phytol.194, 158-67. doi:10.1111/j.1469-8137.2011.04040.x.

Smith AM, Kruger NJ, Lunn JE (2012) Source of sugar nucleotides for starch and cellulose synthesis. Proc. Natl Acad. Sci. USA . 109 E776. doi: 10.1073/pnas.1200878109.

Andriotis V M,  Pike M, Schwarz S. L., Rawsthorne S., Wang TL,, Smith AM (2012) Altered starch turnover in the maternal plant has major effects on Arabidopsis fruit growth and seed composition. Plant Physiol.160, 1175-86. doi: 10.1104/pp.112.205062.

Smith AM (2012) Starch in the Arabidopsis plant. Starch/Staerke64, 421-434. doi: 10.1002/star.201100163.

Crumpton-Taylor M, Grandison S, Png KM, Bushby AJ, Smith AM (2012) Control of starch granule numbers in Arabidopsis chloroplasts. Plant Physiol.158, 905-16. doi:10.1104/pp.111.186957.

Crumpton-Taylor M, Pike M, Lu KJ, Hylton CM, Feil R, Eicke S, Lunn JE, Zeeman SC, Smith AM (2013) Starch synthase 4 is essential for coordination of starch granule formation with chloroplast division during Arabidopsis leaf expansion. New Phytol. 200, 1064-1075. doi: 10.1111/nph.12455.

Howell P, Leigh F, Bates R, Gosman N, Trafford K, Powell W, Smith AM, Greenland A. (2013) Rapid marker-assisted development of advanced recombinant lines from barley starch mutants. Mol. Breed. 33, 243-248. doi: 10.1007/s11032-013-9930-0.

Ruzanski C, Smirnova J, Rejzek M, Cockburn D, Pedersen HL, Pike M, Willats WGT, Svensson B, Steup M, Ebenhoeh O, Smith AM, Field R.(2013) A bacterial glucanotransferase can replace the complex maltose metabolism required for starch to sucrose conversion in leaves at night.  J. Biol. Chem. 288, 28581-28598. doi: 10.1074/jbc.M113.497867.

Scialdone A, Mugford ST, Feike D, Skeffington A, Borrill P, Graf A, Smith AM, Howard M. (2013) Arabidopsis plants perform arithmetic division to prevent starvation at night. eLife 2 e00669. doi: 10.7554/eLife.00669.

Cockburn D, Wilkens C, Ruzanski C, Andersen S, Nielsen JW, Smith AM, Field RA, Willemoes M, Hachem MA, Svensson B. (2014) Analysis of surface binding sites (SBSs) in carbohydrate active enzymes with focus on glycoside hydrolase families 13 and 77 — a mini-review. Biologia 69, 705-712. doi: 10.2478/s11756-014-0373-9.

Howard TP, Fahy B, Leigh F., Howell P., Powell W., Greenland A., Trafford K., Smith AM (2014) Use of advanced recombinant lines to study the impact and potential of mutations affecting starch synthesis in barley. J. Cereal Sci. doi:10.1016/j.jcs.2013.12.012.

Mugford ST, Fernandez O, Brinton J, Flis A, Krohn N, Encke B, Feil R, Sulpice R, Lunn JE, Stitt M, Smith AM (2014) Regulatory Properties of ADP Glucose Pyrophosphorylase Are Required for Adjustment of Leaf Starch Synthesis in Different Photoperiods. Plant Physiol. 166, 1733-47. doi: 10.1104/pp.114.247759.

Vriet C, Smith AM, Wang TL (2014) Root starch reserves are necessary for vigorous re-growth following cutting back in Lotus japonicus. PLOS ONE9 e87333. doi:10.1371/journal.pone.0087333.

Skeffington AW, Graf A, Duxbury Z, Gruissem W, Smith AM (2014) Glucan, water dikinase exerts little control over starch degradation in Arabidopsis leaves at night. Plant Physiol. 165(2), 866-879. doi:10.1104/pp.114.237016

Borrill P, Fahy B, Smith AM, Uauy C (2015) Wheat Grain Filling Is Limited by Grain Filling Capacity rather than the Duration of Flag Leaf Photosynthesis: A Case Study Using NAM RNAi Plants. PLoS ONE 10(8): e0134947. doi:10.1371/journal.pone.0134947.

Andriotis VM, Rejzek M, Rugen MD, SvenssonB, Smith AM, Field RA (2016) Iminosugar inhibitors of carbohydrate-active enzymes that underpin cereal grain germination and endosperm metabolism. Biochem Soc. Trans. 2016 Feb 15: 44(1): 159-65. doi: 10.1042/BST20150222.

Andriotis VM, Saalbach G, Waugh R, Field RA, Smith AM (2016) The Maltase Involved in Starch Mtabolism in Barley Endosperm is Encoded by a Single Gene.PLoS ONE 11 pe0151642. doi: 10.1371/journal.pone.0151642

Feike D, Seung D, Graf A, Bischof S, Ellick T, Coiro M, Soyk S, Eicke S, Mettler-Altman T, Lu K-J, Trick M, Zeeman SC, Smith AM (2016)The starch granule-associated protein EARLY STARVATION1 (ESV1) is required for the control of starch degradation in Arabidopsis thaliana leaves. The Plant Cell tpc.00011.2016. Advance Publication May 20, 2016. http:/​/​dx.​doi.​org/​10.​1105/​tpc.​16.​00011

Andriotis VM, Rejzek M, Barclay E, Rugen MD, Field RA, Smith AM (2016) Cell wall degradation is required for normal starch mobilisation in barley endosperm. Sci Rep 13:6: 33215. http://dx.doi.org/10.1038/srep33215 

Atkinson N., Leitao N., Orr D. J., Meyer M. T., Carmo-Silva E., Griffiths H., Smith A. M., McCormick A. J. (2017) Rubisco small subunits from the unicellular green alga Chlamydomonas complement Rubisco-deficient mutants of Arabidopsis New Phytologist 214(2): 655-667 Publisher’s version: 10.1111/nph.14414