Crop Transformation

BRACT (Biotechnology Resources for Arable Crop Transformation) is a research facility based at the John Innes Centre providing the wider research community with access to a range of crop transformation and genome editing resources, such as;

  • Development of new targeted gene knockout resources: CRISPR/Cas9 technology
  • Training facilities: hosting visitors for hands-on training at the John Innes Centre
  • Constructs: developed specifically for Brassica and the Cereals - the pBRACT series of vectors

The facility operates on a 'not-for-profit', cost recovery basis and supports researchers worldwide.

BRACT services at the John Innes Centre, include;

  • Wheat transformation
  • Barley transformation
  • Brassica oleracea¬†transformation
  • Brassica napus¬†transformation
  • Potato transformation
  • RNA-guided Cas9 mediated genome editing
  • Constructs including design and assembly
  • Training courses
  • Opportunities to develop collaborative projects
  • Transformation protocols

"The BRACT platform has a long history of providing efficient crop transformation to the research community.

Recently we have demonstrated efficient genome editing in barley and Brassica crops and we are now able to provide targeted gene knockouts using RNA-guided Cas9 in addition to other transformation resources"

- Wendy Harwood, Head of Crop Transformation

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