23 August 2017

Hormonal tug-of-war helps plant roots navigate their journey through the soil, new international study finds

A sophisticated mechanism that allows plant roots to quickly respond to changes in soil conditions has been identified by an international research team.

Scientists from the John Innes Centre and Sapienza University, Rome, combined mathematical and computer modelling with molecular genetics to show how roots can regulate their growth via the interactions of two antagonistic hormones, auxin and cytokinin.

As the root grows and meristem cells at the tip continuously divide, they are left behind in relation to the moving root tip. When these cells reach a certain distance from the tip, called the transition position, they stop dividing and instead start elongating until reaching their maximum lengths. 

But how do cells “know” when they have reached the transition position between division and elongation? What signal do they read out?

This, explains Dr Veronica Grieneisen of the John Innes Centre, is down to something called positional information, which is a common feature in all developing organisms:

“Cells, although initially all identical, need to change fate or behaviour according to where they are located in the embryo or organ. This is positional information.”



Image Caption: The balanced cross-talk between auxin and cytokinin positions the auxin minimum (indicated by *). This minimum determines the size of a root's meristem by triggering cells to transit from division to elongation. When cytokinin effects either dominate (left panel) or are reduced (right panel), the auxin minimum is repositioned (*), causing the meristem to either shrink (left panel) or extend (right panel). The hormonal tug-of-war underlying this repositioning takes place through modifications in auxin transport and degradation. 

 

To solve the puzzle the teams of Dr Grieneisen and Dr Stan Marée from the John Innes Centre needed to discover what “positional information” is available at the transition zone to enable cells to know they are at the right location to transit behaviour from dividing (meristem zone) to elongating (elongation zone), and how this information is established and positioned.

Other studies by Dr Grieneisen, Dr Marée and colleagues had shown that the hormone auxin was present at very high levels at the root tip to maintain certain cells as stem cells, and that this was the result of fast dynamics of auxin swirling around due to PINs (proteins that pump auxin through the root).

Their computational work had revealed how these currents of auxin allowed the auxin maximum and its associated gradient to move together with the growing root, providing part of the necessary positional information required to coordinate the meristem zone.

Further recent work, however, showed that auxin does not regulate the transition alone. Antagonistic cross-talk between auxin and another hormone, cytokinin, could both stabilise the size of the meristem zone, and even change it – thus, either stabilizing root growth, or changing its velocity.

Grieneisen and Sabatini joined forces, and together with the Marée lab developed a computerised root model in which the action of cytokinin on auxin transport and breakdown was examined.

They found that cytokinin’s influence generated a very typical pattern of auxin concentrations in the root: as before, with an auxin maximum at the tip, but they noticed that a dip in auxin appeared right at the transition zone of this computational root. This was then confirmed experimentally.

Dr Grieneisen explains: “It’s like a valley between two steep mountains. The cells in the root, as they move over the auxin landscape, transit from high auxin in the meristem into a region of low levels, and then rapidly again experience rises in auxin.”

“By merely sensing relative changes in auxin, cells robustly notice that they have reached the auxin minimum and thus the point of transition, triggering their switch in behaviour.”

Experiments using Arabidopsis roots demonstrated that a tug-of-war between cytokinin and auxin results in this auxin minimum being positioned either closer to the root tip (if cytokinin is “winning” the tug-of-war), or it being positioned further away from the tip (when auxin is “winning” the tug-of-war).

In nature this mechanism allows the root to respond to its environment, proliferating in favourable conditions while restricting growth in adversity.

The findings are published in the paper An Auxin Minimum Triggers the Developmental Switch from Cell Division to Cell Differentiation in the Arabidopsis Root PNAS: http://www.pnas.org/content/early/2017/08/17/1705833114.abstract

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