Interest regarding CRISPR/cas9-mediated gene editing has grown exponentially over the last few years.
Superseding many well-known sequence-specific nucleases like ZFNs and TALENs, CRISPR/cas9-mediated gene editing has the capacity precisely alter plant genomes using either knock-out, knock-in or base-editing technology. Undoubtedly, gene editing is an invaluable tool not only to improve crop characteristics but remains key to aiding our understanding in gene function.
Currently, knock-in efficiencies in monocot systems such as Wheat and Barley are significantly lower compared to knock-outs.
Yvie’s proof of concept project focuses on improving homologous-recombination frequencies which are responsible for effective gene repair, potentially increasing the likelihood of knock-in events.
She aims to induce herbicide resistance into Barley using viral replicons, while working alongside the crop transformation BRACT group.