Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).
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We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolorA3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.