Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis.

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Aj_EasH is a non-heme iron- and a-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.