Arabidopsis Recombinant Inbred populations

These populations are a joint output of the research programme of the Bancroft group in the Department of Crop Genetics and of the Exploiting Natural Variation in Arabidopsis platform technology of the JIC Genome Laboratory.

364 Arabidopsis accessions were screened (O’Neill et al., 2003) and based on these results six Arabidopsis thaliana recombinant inbred populations were developed by single seed descent to F8 (O’Neill et al., 2008).The parents of the populations were originally selected for contrasting seed lipid characteristics. However, as they were also selected as being from diverse geographic origins, the populations are expected to have captured a wide range of genetic diversity for many additional traits. Kondara and Sorbo are both from Tajikistan while Ag-0, Br-0, Ct-1, Ga-0, Gy-0, Mz-0, Nok-3, Ts-5 and Wt-5 are all European in origin and Cvi-0 is from the Cape Verdi Islands. Vernalization of plants at the seedling stage was conducted throughout, as some of the parent accessions had a strong vernalization requirement for flowering.

The six populations are available from the Nottingham Arabidopsis Stock Centre.

JIC Arabidopsis RI populations
Population		Female parent		Male parent		Number of lines
		Accession	NASC Cat.No.	Accession	NASC Cat.No.		NASC Cat.No.
CA		Cvi-0	N902	Ag-0	N936	94	N717140
KB		Kondara	N916	Br-0	N994	94	N717139
NG		Nok-3	N1404	Ga-0	N1180	94	N717142
SG		Sorbo	N931	Gy-0	N1216	94	N717138
TJ		Ts-5	N1558	Mz-0	N9632	94	N717141
WC		Wt-5	N1612	Ct-1	N1094	94	N717137

The genetic linkage maps have been constructed using communal markers. We strongly encourage contribution of marker data from users of the populations to enable these maps to be further developed.

The latest maps for these populations and QTL identified for seed fatty acid composition and oil content are described by O’Neill et al., (2011).

Relevant Publications

Towards the genetic architecture of seed lipid biosynthesis and accumulation in Arabidopsis thaliana.
CM O'Neill, C Morgan, C Hattori, M Brennan, U Rosas, H Tschoep, P X Deng, D Baker, R Wells and I Bancroft (2011) Published online 6th July 2011 in Heredity.

Six new Recombinant Inbred populations for the study of quantitative traits in Arabidopsis thaliana
CM O’Neill, C Morgan, J Kirby, H Tschoep, PX Deng, M Brennan, U Rosas, F Fraser, C Hall, S Gill and I Bancroft. (2008)
Theoretical and Applied Genetics 116:623-634

Natural variation for seed lipid traits in Arabidopsis thaliana
CM O’Neill, S Gill, D Hobbs, C Morgan and I Bancroft (2003)
Phytochemistry 64: 1077-1090