John Innes Centre

Dr Lesley Boyd

Lab Pages

Lab MembersExpand lab members list

Related links

Curriculum Vitae

  • 1980 B.Sc., University College, London, UK
  • 1984 M.Sc., University of Wales, Aberystwyth, Wales, UK
  • 1985 Scientific Officer, Plant Breeding Institute, Cambridge, UK
  • 1990 Ph.D., University of Saskatchewan, Saskatoon, Canada
  • 1994 Post-Doctoral Scientist, John Innes Centre, UK
  • 1995 - present Group Leader, John Innes Centre, UK

Lesley Boyd

Project Leader

Crop Genetics

Contact details

lesley.boyd@jic.ac.uk

Research interests

The work of the research group headed by Dr. Lesley A. Boyd investigates the genetics and biology of cereal fungal pathogen interactions, with past work focusing on the wheat-rust interaction (Boyd, 2005).

A major focus of the group has been the genetic and biological dissection of yellow rust (also known as stripe rust) and more recently stem rust resistance in wheat. National and international programs have characterised the genetic biodiversity of yellow rust resistance in national wheat germplasm collections. Microscopic studies have examined the biology of the wheat-yellow rust interaction, with particularly focus on durable sources of resistance in wheat (Boyd, 2006).

New areas of research include the genetics and biology of non-host resistance, using the wheat-Magnaporthe grisea, Puccinia triticina and Blumeria graminis systems, and Systemic Acquired Resistance (SAR) in cereals, modelled on DIR1-mediated SAR found in Arabidopsis (www.jic.ac.uk/staff/lesley-boyd/index.htm).

Much of the work undertaken in the group of Dr. Lesley A. Boyd has involved collaborations with scientists from less developed countries. JIC is keen to support and mentor science and scientists from less developed countries and programs are being established that will allow an expansion of Lelsey Boyd's involvement in collaborative projects that support international development.

Genetic biodiversity of rust resistance in wheat

National and international programs have characterised sources of partial, field resistance and race-specific R-gene resistance to yellow and stem rust in wheat. Programs have included genetic analysis of adult plant resistance in UK, European, Turkish and South African wheat cultivars.

PCR-based markers developed for the yellow rust, race-specific R-genes, Yr5 and Yr10 have provided tools for marker assisted selection breeding and are currently being used to stack these R-genes, along with partial QTLs for yellow rust resistance into French wheat cultivars as part of a European Union Framework 6 Initiative.

Biology of the wheat-yellow rust interaction

A major interest of the group has been the characterisation of the microphenotypes of durable sources of yellow rust resistance in wheat. This has been facilitated by the development within the group of a light microscopy procedure that allows examination of yellow rust development in adult plant tissues (Garrood, 2001; Melichar, 2006). Developmental arrest of the fungus is examined in relation to host cell death and the generation of reactive oxygen intermediates.

Non-host resistance in wheat

An international collaborative project, Cereal Immunity, funded by the CGIAR Generation Challenge Program (www.generationcp.org) involves Agroplis (France), INRA (France), JIC (UK), UCD (USA), NIAS (Japan), CIMMYT (Mexico) and Embrapa (Brazil). Through global, gene expression screens in rice and wheat, Magnaporthe grisea and Puccinia triticina interactions, candidate non-host resistance genes have been identified.

Systemic aquired resistance in cereals

DIR1-mediated SAR, characterised in Arabidopsis thaliana, is being used as the starting point to study SAR in cereals. This program started in October 2005 and addresses the transfer of fundamental research in model plant species into crops.

Selected Publications

Agenbag G. M., Pretorius Z. A., Boyd L. A., Bender C. M., Prins R. (2012)
Identification of adult plant resistance tostripe rust in the wheat cultivar Cappelle-Desprez
Theoretical and Applied Genetics online early
DOI:DOI 10.1007/s00122-012-1819-5
Colebrook E. H., Creissen G., McGrann G. R., Dreos R., Lamb C., Boyd L. A. (2012)
Broad-spectrum acquired resistance in barley induced by the Pseudomonas pathosystem shares transcriptional components with Arabidopsis SAR.
Molecular Plant-Microbe Interactions 25 (5) 658-667
DOI:10.1094/MPMI-09-11-0246
Tufan H. A., McGrann G. R., Maccormack R., Boyd L. A. (2012)
TaWIR1 contributes to post-penetration resistance to Magnaporthe oryzae, but not Blumeria graminis f. sp. tritici, in wheat.
Molecular Plant Pathology
DOI:10.1111/j.1364-3703.2011.00775.x
Jagger L. J., Newell C., Berry S. T., Maccormack R., Boyd L. A. (2011)
The genetic characterisation of stripe rust resistance in the German wheat cultivar Alcedo.
Theoretical and Applied Genetics 122 (4) 723-33
DOI:10.1007/s00122-010-1481-8
Jagger L. J., Newell C., Berry S. T., MacCormack R., Boyd L. A. (2011)
Histopathology provides a phenotype by which to characterise stripe rust genes in wheat
Plant Pathology 60 (4) 640-648
DOI:10.1111/j.1365-3059.2011.02436.x
Sayar-Turet M., Dreisigacker S., Braun H. J., Hede A., MacCormack R., Boyd L. A. (2011)
Genetic variation within and between winter wheat genotypes from Turkey, Kazakhstan anad Europe as determined by nucleotide-binding-site profiling
Genome 54 (5) 419-430
Tufan H. A., Stefanato F., McGrann G. R. D., MacCormack R., Boyd L. (2011)
The barley stripe mosaic virus system used for virus induced gene silencing in cereals differentially affects susceptibility to fungal pathogens in wheat
Journal of Plant Physiology 168 (9) 990-994
DOI:10.1016/j.jplph.2010.11.019
Bozkurt T. O., McGrann G. R. D., MacCormack R., Boyd L. A., Akkaya M. S. (2010)
Cellular and transcriptional responses of wheat during compatible and incompatible race-specific interactions with Puccinia striiformis f. sp. tritici
Molecular Plant Pathology 11 (5) 625-640
DOI:10.1111/j.1364-3703.2010.00633
Tufan H. A., McGrann G. R., Magusin A., Morel J. B., Miché L., Boyd L. A. (2009)
Wheat blast: histopathology and transcriptome reprogramming in response to adapted and nonadapted Magnaporthe isolates.
New Phytologist 184 (2) 473-484
DOI:10.1111/j.1469-8137.2009.02970.x
Melichar J. P., Berry S., Newell C., Maccormack R., Boyd L. A. (2008)
QTL identification and microphenotype characterisation of the developmentally regulated yellow rust resistance in the UK wheat cultivar Guardian.
Theoretical and Applied Genetics 117 (3) 391-399
DOI:10.1007/s00122-008-0783-6
Smith P. H., Hadfield J., Hart N. J., Koebner R. M. D., Boyd L. A. (2007)
STS markers for the wheat yellow rust resistance gene Yr5 suggest a NBS-LRR-type resistance gene cluster
Genome 50 259-265
DOI:10.1139/G07-004
Boyd L. A. (2006)
Perspective: can the durability of resistance be predicted?
Journal of the Science of Food and Agriculture 86 2523-2526
DOI:10.1002/jsfa.2648
Boyd L. A. (2005)
Can robigus defeat an old enemy? - Yellow rust of wheat
Journal of Agricultural Science 143 233-243
DOI:10.1017/S0021859605005095

Recent Publications

Wellings C. R., Boyd L. A., Xianming C. M. (2012)
Resistance to stripe rust in wheat: Pathogen biology driving resistance breeding
Disease Resistance in Wheat
CABI
(4) 63-83