Research Assistant (Webster Group)

We’re looking for a Research Assistant to join the Webster Group to work on cutting-edge science in the field of biochemistry and metabolism.

Research in the Webster group focuses on understanding the molecular machines that express photosynthetic genes. The group aims to discover what determines the timing and level of photosynthetic proteins production.

The role

Photosynthesis underpins oxygenation of the atmosphere and supports the global foodchain. In plants it occurs within chloroplasts, which contain a genome that encodes core subunits of the photosynthetic complexes. Despite the importance of chloroplasts, we lack a detailed understanding of how chloroplast genes are expressed.

The Research Assistant role will establish methods for the recombinant expression of protein complexes that perform gene expression in chloroplasts. Modern cloning toolkits developed for synthetic biology will be used to assemble multi-gene expression plasmids, and intact protein complexes will be purified for use in biochemical and cryo-EM analyses.

This project is an essential foundation of our goal to understand the roles of proteins that underpin photosynthesis in plants. The work will support the long-term effort to control photosynthetic output and timing for improved crops and biotechnologies. They will also shed new light on the nature of the poorly-understood machinery of chloroplast gene expression.

In this role, you will have the opportunity to develop your skills in modern DNA cloning methods, such as Gibson assembly and Golden Gate cloning, recombinant protein expression, and protein purification techniques, such as chromatography.

You will also support world leading scientists who are experts in their field.

The ideal candidate

You will have a BSc or equivalent in molecular biology or a similar discipline and an understanding of recombinant protein expression. Demonstrable experience in protein purification methods and experience in DNA cloning for recombinant protein expression are desirable.

The ideal candidate should be available to start as soon as possible and by August 2022.

Additional information

Interviews will be held on 13th June 2022.

This role does not meet the full salary requirements set by UKVI to allow for visa sponsorship.  However, some individuals may still be eligible for visa sponsorship depending on their personal circumstances.  If you require visa sponsorship, please click here to find out if you qualify before applying. Please note, the occupation code for this role is 2112, which is on the shortage occupation list.

Further information and details of how to apply can be found here,  or contact the Human Resources team on 01603 450462 or nbi.recruitment@nbi.ac.uk quoting reference 1004270.

We are an equal opportunities employer, actively supporting inclusivity and diversity.  As a Disability Confident organisation, we guarantee to offer an interview to all disabled applicants who meet the essential criteria for this vacancy. We are proud to hold a prestigious Gold Athena SWAN award in recognition of our inclusive culture, commitment and good practices towards advancing of gender equality. We offer an exciting, stimulating, diverse research environment and actively promote a family friendly workplace. The Institute is also a member of Stonewall’s Diversity Champions programme.

The John Innes Centre is a registered charity (No. 223852) grant-aided by the Biotechnology and Biological Sciences Research Council.

Apply for this role

More Vacancies