Nuclear Magnetic Resonance

Nuclear Magnetic Resonance (NMR) encompasses several powerful structural and analytical methodologies which are capable of providing atomic level information about molecules.

For example, NMR can determine the numbers of particular atoms within a molecule, their chemical environments and how they are interconnected and spatially arranged based on their magnetic properties.

High-resolution NMR spectrometers for structural elucidation and analysis

Within the facility there are 3 spectrometers dedicated to the routine analysis of small and medium sized molecules, two operating at 400 Mhz and another at 500 MHz.

All 3 instruments are dual channel and have variable temperature capabilities.

They are configured with autosamplers for medium throughput analysis and broad band tuneable probes enabling observation of the widest range of nuclei.

They are programmed with a wide variety of NMR techniques in order to fully characterise and describe the systems under investigation.

"We support the science at the John Innes Centre with NMR Spectroscopy for structural analysis of new compounds, metabolite identification, monitoring enzymatic reactions and understanding enzyme specificity and mode of action"

- Sergey Nepogodiev, NMR Spectroscopy

The Nuclear Magnetic Resonance equipment and services at the John Innes Centre includes;

  • Bruker 400 MHz Nuclear Magnetic Resonance spectrometer
  • The instrument is particularly suitable for analyses of low and medium molecular weight compounds (2 to 10 mg quantities). For lower concentration long acquisition times can be arranged
  • 1H NMR and 13C NMR spectroscopy is routinely used in automated mode for recording both 1D and 2D spectra
  • A wide range of other Nuclear Magnetic Resonance experiments is available in fully automated mode
  • Many other experiments can be carried out using manual setup, including long acquisition time, kinetic measurements, variable temperature experiments etc
  • The instrument can also be used for 19F, 31P and other atoms nuclei NMR spectra recording

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