John Innes Centre site search
George Lomonossoff
Biological Chemistry Department
Rob Field


Aljabali A. A., Barclay J. E., Steinmetz N. F., Lomonossoff G. P., Evans D. J.
Controlled immobilisation of active enzymes on the cowpea mosaic virus capsid.
(2012) 4 5640-5

Sainsbury F., Saxena P., Geisler K., Osbourn A., Lomonossoff G.
Using a virus-derived system to manipulate plant natural product biosynthetic pathways
Methods in Enzymology: Natural Product Biosynthesis by Microorganisms and Plants, Part C
(Editors: Hopwood D.) Elsevier (2012) Vol. 517 185-202

Vardakou M., Sainsbury F., Rigby N., Mulholland F., Lomonossoff G. P.
Expression of active recombinant human gastric lipase in Nicotiana benthamiana using the CPMV-HT transient expression system
Protein Expression and Purification (2012) 81 (1) 69-74


Aljabali A. A. A., Barclay J. E., Cespedes O., Rashid A., Staniland S. S., Lomonossoff G. P., Evans D. J.
Charge modified Cowpea mosaic virus particles for templated mineralization.
Advanced Functional Materials (2011) 21 (21) 4137-4142

Aljabali A. A. A., Lomonossoff G. P., Evans D. J.
CPMV-polyelectrolyte-templated gold nanoparticles.
Biomacromolecules (2011) 12 (7) 2723-2728

Montague N. P., Thuenemann E. C., Saxena P., Saunders K., Lenzi P., Lomonossoff G. P.
Recent advances of Cowpea mosaic virus-based particle technology.
Human Vaccine (2011) 7 (3) 383-90

Sainsbury F., Saunders K., Aljabali A. A. A., Evans D. J., Lomonossoff G. P.
Peptide-controlled access to the interior surface of empty virus nanoparticles.
ChemBioChem (2011) 12 (16) 2435-2440

Saxena P., Hsieh Y. C., Alvarado V. Y., Sainsbury F., Saunders K., Lomonossoff G. P., Scholthof H. B.
Improved foreign gene expression in plants using a virus-encoded suppressor of RNA silencing modified to be developmentally harmless.
Plant Biotechnology Journal (2011) 9 (6) 703-12

Sohn S. H., Choi M. S., Kim K. H., Lomonossoff G.
The epigenetic phenotypes in transgenic Nicotiana benthamiana for CaMV 35S-GFP are mediated by spontaneous transgene silencing.
Plant Biotechnology Reports (2011) 5 273-281

Sohn S. H., Huh S. M., Kim K. H., Park J. W., Lomonossoff G.
Effect of Rice Stripe virus NS3 on transient gene expression and transgene co-silencing.
Plant Pathology Journal (Korea) (2011) 27 (4) 310-314

Sun Q. Y., Ding L. W., Lomonossoff G. P., Sun Y. B., Luo M., Li C. Q., Jiang L., Xu Z. F.
Improved expression and purification of recombinant human serum albumin from transgenic tobacco suspension culture.
Journal of Biotechnology (2011) 155 (2) 164-72

Aljabali AAA., Shah SN, Evans-Gowing R, Lomonossoff GP, Evans DJ.
Chemically-coupled-peptide promoted virus nanoparticle templated mineralization.
Integrative Biology (2011) 3 119-125


Aljabali AAA, Barclay JE, Lomonossoff GP, Evans DJ.
Virus templated metallic nanoparticles.
Nanoscale (2010) 2 2596-2600

Aljabali AAA, Barclay JE, Butt JN, Lomonossoff GP, Evans DJ.
Redox-active ferrocene-modified Cowpea mosaic virus nanoparticles.
Dalton Transactions (2010) 39 7569-7574

Aljabali AAA, Sainsbury F, Lomonossoff GP, Evans DJ.
Cowpea mosaic virus unmodified empty viruslike particles can be loaded with metal and metal oxide.
Small (2010) 6 818-821


Saunders K, Sainsbury F, Lomonossoff GP.
Efficient generation of cowpea mosaic virus empty virus-like particles by the proteolytic processing of precursors in insect cells and plants.
Virology (2009) 393 329-37

Mugford ST, Qi X, Bakht S, Hill L, Wegel E, Hughes RK, Papadopoulou K, Melton R, Philo M, Sainsbury F, Lomonossoff GP, Roy AD, Goss RJ, Osbourn A.
A serine carboxypeptidase-like acyltransferase is required for synthesis of antimicrobial compounds and disease resistance in oats.
Plant Cell (2009) 21 2473-2484

Shah SN, Steinmetz NF, Aljabali AAA, Lomonossoff GP, Evans DJ.
Environmentally benign synthesis of virus-templated, monodisperse, iron-platinum nanoparticles.
Dalton Transactions (2009) Part 40 8479-8480

Meshcheryakova YA, Eldarov MA, Migunov AI, Stepanova LA, Repko IA, Kiselev CI, Lomonossoff GP, Skryabin KG.
Cowpea mosaic virus chimeric particles bearing the ectodomain of matrix protein 2 (M2E) of the influenza A virus: production and characterization.
Molecular Biology (2009) 43 694-703

Sainsbury F, Thuenemann EC, Lomonossoff GP.
pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants.
Plant Biotechnology Journal (2009) 7 682-693

Steinmetz NF, Shah SN, Barclay JE, Rallapalli G, Lomonossoff GP, Evans DJ
Virus-templated silica nanoparticles
Small (2009) 5 813-816

Sainsbury F, Liu L, Lomonossoff GP.
Cowpea mosaic virus-based systems for the expression of antigens and antibodies in plants.
Methods in Molecular Biology (Editors: Faye L.) Humana Press Inc. (2009) 483 25-39


Sainsbury F, Lomonossoff GP.
Extremely high-level and rapid transient protein production in plants without the use of viral replication
Plant Physiology (2008) 148 1212-1218

Choi MS, Yoon IS, Rhee Y, Choi SK, Lim SH, Won SY, Lee YH, Choi HS, Lee SC, Kim KH, Lomonossoff GP,
Sohn SH.
The effect of Cucumber mosaic virus 2b protein to transient expression and transgene silencing mediated by agro-infiltration
Plant Pathology (2008) 24 296-304

Steinmetz N. F., Bize A., Findlay K. C., Lomonossoff G. P., Manchester M., Evans D. J., Prangishvili D.
Site-specific and spatially controlled addressability of a new viral nanobuilding block: Sulfolobus islandicus rod-shaped virus 2
Advanced Functional Materials (2008)18 3478-3486

Meshcheriakova IuA, El'darov MA, Beales L, Skriabin KG, Lomonossoff GP.
Production of hepatitis B virus core particles protein in plants, by using cowpea mosaic virus-based vector
Vopr Virusol. 2008 May-Jun;53(3):15-20. Russian.

Steinmetz NF, Findlay KC, Noel TR, Parker R, Lomonossoff GP, Evans DJ.
Layer-by-layer assembly of viral nanoparticles and polyelectrolytes: the film architecture is different for spheres versus rods.
Chembiochem. 2008 Jul 2;9(10):1662-70.

Liu L, Lomonossoff GP.
Site-directed mutagenesis of whole viral genomes.
Methods Mol Biol. 2008;451:395-404.

Steinmetz NF, Bock E, Richter RP, Spatz JP, Lomonossoff GP, Evans DJ.
Assembly of multilayer arrays of viral nanoparticles via biospecific recognition: a quartz crystal microbalance with dissipation monitoring study.
Biomacromolecules. 2008 Feb;9(2):456-62.

Sainsbury F, Lavoie PO, D'Aoust MA, Vézina LP, Lomonossoff GP.
Expression of multiple proteins using full-length and deleted versions of cowpea mosaic virus RNA-2.
Plant Biotechnol J. 2008 Jan;6(1):82-92.


King DP, Montague N, Ebert K, Reid SM, Dukes JP, Schädlich L, Belsham GJ, Lomonossoff GP.
Development of a novel recombinant encapsidated RNA particle: evaluation as an internal control for diagnostic RT-PCR.
J Virol Methods. 2007 Dec;146(1-2):218-25.

Steinmetz NF, Evans DJ, Lomonossoff GP.
Chemical introduction of reactive thiols into a viral nanoscaffold: a method that avoids virus aggregation.
Chembiochem. 2007 Jul 9;8(10):1131-6.


Monger W, Alamillo JM, Sola I, Perrin Y, Bestagno M, Burrone OR, Sabella P, Plana-Duran J, Enjuanes L, Garcia JA, Lomonossoff GP.
An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus infection when supplied orally in crude plant extracts.
Plant Biotechnol J. 2006 Nov;4(6):623-31.

Steinmetz NF, Lomonossoff GP, Evans DJ.
Decoration of cowpea mosaic virus with multiple, redox-active, organometallic complexes.
Small. 2006 Apr;2(4):530-3.

Cañizares MC, Liu L, Perrin Y, Tsakiris E, Lomonossoff GP.
A bipartite system for the constitutive and inducible expression of high levels of foreign proteins in plants.
Plant Biotechnol J. 2006 Mar;4(2):183-93.

Steinmetz NF, Calder G, Lomonossoff GP, Evans DJ.
Plant viral capsids as nanobuilding blocks: construction of arrays on solid supports.
Langmuir. 2006 Nov 21;22(24):10032-7.

Alamillo JM, Monger W, Sola I, García B, Perrin Y, Bestagno M, Burrone OR, Sabella P, Plana-Durán J, Enjuanes L, Lomonossoff GP, García JA.
Use of virus vectors for the expression in plants of active full-length and single chain anti-coronavirus antibodies.
Biotechnol J. 2006 Oct;1(10):1103-11.

Steinmetz NF, Lomonossoff GP, Evans DJ.
Cowpea mosaic virus for material fabrication: addressable carboxylate groups on a programmable nanoscaffold.
Langmuir. 2006 Apr 11;22(8):3488-90.

Mechtcheriakova IA, Eldarov MA, Nicholson L, Shanks M, Skryabin KG, Lomonossoff GP.
The use of viral vectors to produce hepatitis B virus core particles in plants.
J Virol Methods. 2006 Jan;131(1):10-5.


Cañizares MC, Lomonossoff GP, Nicholson L.
Development of cowpea mosaic virus-based vectors for the production of vaccines in plants.
Expert Rev Vaccines. 2005 Oct;4(5):687-97.

Cañizares MC, Nicholson L, Lomonossoff GP.
Use of viral vectors for vaccine production in plants.
Immunol Cell Biol. 2005 Jun;83(3):263-70.

Liu L, Cañizares MC, Monger W, Perrin Y, Tsakiris E, Porta C, Shariat N, Nicholson L, Lomonossoff GP.
Cowpea mosaic virus-based systems for the production of antigens and antibodies in plants.
Vaccine. 2005 Mar 7;23(15):1788-92.


Cañizares MC, Taylor KM, Lomonossoff GP.
Surface-exposed C-terminal amino acids of the small coat protein of Cowpea mosaic virus are required for suppression of silencing.
J Gen Virol. 2004 Nov;85(Pt 11):3431-5.

Liu L, Grainger J, Cañizares MC, Angell SM, Lomonossoff GP.
Cowpea mosaic virus RNA-1 acts as an amplicon whose effects can be counteracted by a RNA-2-encoded suppressor of silencing.
Virology. 2004 May 20;323(1):37-48.


Yasawardene SG, Lomonossoff GP, Ramasamy R.
Expression & immunogenicity of malaria merozoite peptides displayed on the small coat protein of chimaeric cowpea mosaic virus.
Indian J Med Res. 2003 Sep;118:115-24.

Porta C, Spall VE, Findlay KC, Gergerich RC, Farrance CE, Lomonossoff GP.
Cowpea mosaic virus-based chimaeras. Effects of inserted peptides on the phenotype, host range, and transmissibility of the modified viruses.
Virology. 2003 May 25;310(1):50-63.


Chatterji A, Burns LL, Taylor SS, Lomonossoff GP, Johnson JE, Lin T, Porta C.
Cowpea mosaic virus: from the presentation of antigenic peptides to the display of active biomaterials.
Intervirology. 2002;45(4-6):362-70.

Porta C, Lomonossoff GP.
Viruses as vectors for the expression of foreign sequences in plants.
Biotechnol Genet Eng Rev. 2002;19:245-91.

Blanch EW, Hecht L, Syme CD, Volpetti V, Lomonossoff GP, Nielsen K, Barron LD.
Molecular structures of viruses from Raman optical activity.
J Gen Virol. 2002 Oct;83(Pt 10):2593-600.

John Innes Centre