Biomolecular Analysis

Protein Crystallography

Our protein crystallography platform provides facilities and expertise to help determine the three-dimensional structure of proteins (or macromolecules) starting from a purified sample.

The team stock a range of pre-aliquoted screens, for hassle-free setup of crystallisation trials via crystallisation robots. The platform also has a fully automated Plate Imager which will take regular images of experiments enabling rapid identification of suitable crystallisation conditions.

They also have regular access to macromolecular crystallography beamlines at the Diamond Light Source for X-ray data collection. The team also arrange the shipping of samples to Diamond, assist with data collection and manage all the subsequent data curation.

In addition, together with the Bioimaging Platform, they support macromolecular structure determination using cryo-electron microscopy (cryo-EM).

The Protein Crystallography platform is able to take on both academic and commercial structural biology projects.

We recommend contacting the team before beginning protein expression, as they can offer advice on construct design, sample quality and sample quantity. They can also advise on protein purification.

Depending on the level of user expertise, they’re available to help with any of the subsequent steps necessary to determine your structure.

There are three main modes of operation available;

  • Trained users do their own experiments
  • Platform staff perform the work
  • A mixture of the above (which may include user training)

If you are interested in using the platform, contact David Lawson or Clare Stevenson to discuss what you would like to achieve. We will offer advice on likely costs, timescales and feasibility.