Biomolecular Analysis

Chemistry

Our Chemistry platform provides expertise and staff training in various aspects of organic chemistry to scientists across the Norwich Research Park, and commercial partners.

The team, in our state-of-the-art laboratory can offer;

  • Infrastructure for chemical synthesis or custom synthesis
  • Compound purification
  • Compound characterisation (with NMR, Proteomics and Metabolomics)
  • Natural product isolation
  • Structure elucidation (with NMR)
  • Biotransformation
  • Bioconjugation
  • Enzyme assay development
  • Infrastructure for safe handling and work with organic solvents and other hazardous chemicals
  • Complex analytical sample preparation requiring organic solvent extraction, solvent partitioning or solid phase extraction (with Metabolomics)
  • Synthesis of internal standards or target analytes as reference for analysis (with Metabolomics)

The Chemistry Platform equipment and infrastructure at the John Innes Centre, include;

  • Dionex Ultimate 3000 HPLC, analytical or semi-preparative, equipped with UV/vis or charged aerosol detectors
  • Thermo Dionex Ultimate 3000 HPLC, preparative, with UV/vis
  • Thermo Dionex ICS-5000 equipped with pulsed amperometric detector (PAD) for carbohydrate analysis by High-Performance Anion-Exchange chromatography with Pulsed Amperometric Detection (HPAE-PAD)
  • Thermo Dionex ICS-3000 Ion Chromatography with UV/vis
  • Advion Expression Compact Mass Spectrometer with ESI or APCI ionisation sources
  • Perkin Elmer Fourier Transform Infrared (FT-IR) spectrometer with Attenuated Total Reflection (ATR) attachment
  • Biotage Isolera high performance flash purification system
  • Polarimeter
  • Bϋchi Rotatory Evaporators
  • Genevac EZ-2 Evaporator
  • Labconco Freeze dryer
  • Thermo Savant SpeedVac Concentrator SC 210A
  • Varian Cary 50 Bio UV-vis Spectrophotometer

The Chemistry Platform equipment and services are available at the hourly rate indicated below.

  • Staff = £45
  • Thermo Dionex Ultimate 3000 HPLC analytical, semi-preparative, with UV/vis or CAD = £18
  • Thermo Dionex Ultimate 3000 HPLC preparative with UV/vis = £0
  • Thermo Dionex ICS-5000-PAD = £18
  • Thermo Dionex ICS-3000 Ion Chromatography with UV/vis = £7
  • Advion Compact Mass Spectrometer with ESI or APCI = £20
  • Perkin Elmer FT-IR with ATR = £5
  • Biotage Isolera = £6

The Platform infrastructure is available to all trained staff and visitors at no additional cost.