Biophysical Analysis

Circular Dichroism Spectroscopy

Circular Dichroism Spectroscopy allows us to use light to investigate the structural properties of biological molecules such as proteins, DNA and RNA.

Circular Dichroism Spectroscopy is most often used to:

  • Examine the folding status of proteins in solution
  • Provide information on the percentage of each type of secondary structure, e.g. α-helix and β-sheets, in your protein of interest
  • Detect changes in structure during protein-protein or protein-ligand binding events

The Biophysical Analysis platform has a Chirascan Plus Spectrophotometer (Applied Photophysics) which uses a Xenon light source to collect CD data on biological macromolecules in solution at near and far UV wavelengths.

Training is required before using the instrument, and this is provided on request by Biophysical Analysis Platform staff.

The biophysical analysis team run frequent training sessions on all techniques.