Iron transport in the legume-Rhizobium symbiosis

To monitor iron delivery to the bacterial symbionts housed in root nodules, the Balk lab has developed an iron-responsive reporter.

The bacterial lux (luciferase) operon was placed under the control of the mbfA promoter (membrane-bound ferritin). This promoter is active during nodule development and regulated by iron.

Using the iron-responsive reporter, we have analysed mutants of the host plant Medicago truncatula to see if a particular plant gene is required for iron delivery to the symbiont (Walton JW et al., manuscrpt in preparation).

Iron transport in the legume-Rhizobium symbiosis
Iron transport in the legume-Rhizobium symbiosis