Diversity in Pisum

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This growing demonstration shows a representation of evolutionary genetic relatedness within Pisum based on a test array of 56 accessions selected to represent the maximum diversity of Pisum. The lines were analysis using DNA markers (101 AFLP and 276 SSAPs) and the results presented in the form of a ‘phylogenetic tree’ or ‘evolutionary tree’. This type of representation groups together lines on the basis of their genetic relatedness with the lengths of branches directly scaled to reflect the genetic distance between any two accessions.

The tree structure is scaled up in a 10m2 grid and transcribed onto cultivated ground using coloured twine with plants of each accession being grown at the end of the relevant branch for that line. Due to the fact that the test array included both wild and cultivated forms, seeds of all lines were chipped and sown in small pots in the glasshouse to ensure even germination. On emergence the seedlings are transferred to cold frames to be hardened off before being planted out in the field. Six seedlings are transplanted in a circle with 10cm circumference and a 1m cane placed to mark the end of each line of the phylogenetic tree. The whole of the demonstration area is kept clear during the growing season by regular hoeing of emerging weeds.

What is immediately clear when viewing this demonstration is how well the different recognised taxa within Pisum are separated by the DNA analysis. The shared morphological features of each taxa are clearly seen in growing plants as are some branches that relate to ecogeographic factors such as isolation and adaptation in cultivated types (Pisum sativum). The best time to come and view this demonstration and to be able to see the features at close hand is as the material comes into flowering in the middle of May.

A clickable digital resource of all the accessions photographed at flowering is be prepared as an information resources which is planned for release at the end of 2012.

There are benefits to growing and observing this diversity set out each year and useful data has been collected which feature in the following publications.

Ambrose, M.J. and Ellis, T.H.N. (2008). Ballistic seed dispersal and associated seed shadow in wild Pisum germplasm. Pisum Genetics v40: 5-10. (http://hermes.bionet.nsc.ru/pg/40/5.htm )

Ambrose, MJ (2010) Field quantification of foliar chlorophyll content in Pisum germplasm. Pisum Genetics v42: 7-10. (http://hermes.bionet.nsc.ru/pg/42/7.htm)

Listing of accessions in the demonstration.


Accession No.

NAME

Country of Origin

2

 P. ABYSSINICUM

ETH

45

 P.TRANSCAUCASICUM

GEO

49

 P.SATIVUM-AFGHANISTAN

AFG

52

 P. ASIATICUM

64

 P. ELATIUS

ISR

85

 P.SATIVUM-AFGHANISTAN

AFG

95

 P.SATIVUM-AFGHANISTAN

AFG

102

 P.SATIVUM-AFGHANISTAN

AFG

109

 P.SATIVUM-AFGHANISTAN

AFG

130

 P. ABYSSINICUM

ETH

156

 P.SATIVUM-SUDAN

SDN

157

 P.SATIVUM-SUDAN

SDN

159

 P.SATIVUM-ETHIOPIA

ETH

181

 KEERAU PEA

NPL

185

 WIRAIG

SDN

188

 WIRAIG

SDN

189

 WIRAIG

SDN

196

 P.SATIVUM-GEORGIA

GEO

199

 P. ELATIUS

ISR

201

 P.THEBAICUM

209

 P.SATIVUM ARVENSE

IND

224

 P. FULVUM

ISR

225

 P. ABYSSINICUM

ETH

241

 P. HUMILE

ISR

250

 P. JOMARDII

EGY

254

 P. ELATIUS

ETH

261

 P. ELATIUS

TUR

262

 P. ELATIUS

TUR

281

 P.SATIVUM-ETHIOPIA

ETH

284

 P.SATIVUM-AFGHANISTAN

AFG

399

 CENNIA

711

 AUSTRIAN WINTER

804

 P.TIBETANICUM

1006

 P. FULVUM

ISR

1010

 P. FULVUM

ISR

1030

 P.SATIVUM-IRAN

IRN

1033

 P.SATIVUM-INDIA

IND

1074

 P. ELATIUS

TUR

1089

 P. ELATIUS

TUR

1092

 P. ELATIUS

GRC

1093

 P. ELATIUS

GRC

1096

 P. ELATIUS

GRC

1346

 P.SATIVUM-MONGOLIA

MNG

1428

 P.TIBETANICUM

TIB

1556

 P. ABYSSINICUM

ETH

1794

 P. HUMILE

ISR

1796

 P. FULVUM

ISR

1846

 P.SATIVUM VAR. HIBERNICUM

GRC

1854

 P. HUMILE

ISR

2055

 P. ELATIUS

ITA

2201

 P. ELATIUS

RUS

2385

 PISUM SP.-YEMEN

YEM

2545

 P. SATIVUM-PAKISTAN

PAK

2546

 P. TRANSCAUCASICUM

GEO

2547

 P. TRANSCAUCASICUM

GEO

2713

 P. CINEREUM

RUS