Dr Giulia Pergolizzi
After training as an organic chemist in Italy, I continued my studies at UEA and King's College London in the UK and received PhD degree in Pharmacy in 2012. Since I joined Prof. Rob Field group at JIC as a PDRA in 2013, I worked on a variety of projects associated with carbohydrate chemistry and biology. To tackle the problem of efficacy of anti-Burkholderia vaccine I worked on preparation of semi-synthetic glycoconjugates based on capsular polysaccharide (CPS) of the bacterium, CPS fragments and several types of carriers including virus-like particles (VLP).
More recently, I have been involved in a large collaborative research programme between several academic and industrial partners which is aimed at the identification and characterization of new glycoenzymes with desirable bioactivities for industrial application in diagnostics, nutraceuticals, biofuels, etc. Thousands of carbohydrate-active enzymes are known to be involved in biosynthesis and metabolism of glycans and glycoconjugates but only limited number of these enzymes found wide-spread industrial applications. My research focus is on the biochemical characterization of glycoenzymes and investigation of their use in the production of known glycans in a cheaper and greener way, as well as the development of new glycomaterials and their characterization. Synthetic organic chemistry and enzymatic synthesis, bioconjugation and biochemical analysis, NMR spectroscopy and mass spectrometry are the main areas of my expertise which let me progress with my research.
ContactTel: int. 2705
Cellodextrin phosphorylase from Ruminiclostridium thermocellum: X-ray crystal structure and substrate specificity analysis
Carbohydrate Research 451 p118-132
Publisher’s version: 10.1016/j.carres.2017.07.005
Structural characterisation of the capsular polysaccharide expressed by Burkholderia thailandensis strain E555:: wbiI (pKnock-KmR) and assessment of the significance of the 2-O-acetyl group in immune protection.
Carbohydrate Research 452 p17-24
Publisher’s version: 10.1016/j.carres.2017.09.011