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Dr Stephen Bornemann

Biological Chemistry Department, John Innes Centre
Norwich Research Park, Norwich NR4 7UH, United Kingdom
stephen.bornemann@bbsrc.ac.uk
Tel
+44 (0)1603 450741 Fax +44 (0)1603 450018

enzymes and small molecules of plants and microbes

Selected Steph Bornemann Lab Publications
with links to NCBI PubMed or journal web site

Detection of enzyme-catalyzed polysaccharide synthesis on surfaces
Clé C, Martin C, Field RA, Kuzmič P and Bornemann S
Biocatal. Biotrans. 28, 64-71 (2010)

The pH-dependent structures of the manganese binding sites in oxalate decarboxylase as revealed by high-field electron paramagnetic resonance
Tabares LC, Gätjens J, Hureau C, Burrell MR, Bowater L, Pecoraro VL, Bornemann S and Un S
J Phys Chem B 113, 9016-9025 (2009)

Detection of transglucosidase-catalyzed polysaccharide synthesis on a surface in real-time using surface plasmon resonance spectroscopy
Clé C, Gunning AP, Syson K, Bowater L, Field RA and Bornemann S
J Am Chem Soc 130, 15234-15235 (2008)
pdf Free access after registration with this link

Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity
Rausch G, Ehammer H, Bornemann S and Macheroux P
FEBS J 275, 1464-1473 (2008)

Oxalate decarboxylase and oxalate oxidase activities can be interchanged with a specificity switch of up to 282,000 by mutating an active site lid
Burrell MR, Just VJ, Bowater L, Fairhurst SA, Requena L, Lawson DM and Bornemann S
Biochemistry 46, 12327-12336 (2007)
pdf Free access after registration with this link

The identity of the active site of oxalate decarboxylase and the importance of the stability of active site lid conformations
Just VJ, Burrell MR, Bowater L, McRobbie I, Stevenson CEM, Lawson DM and Bornemann S
Biochem J 407, 397-406 (2007)

Mutagenic analysis of an invariant aspartate residue in chorismate synthase supports its role as an active site base
Rauch G, Ehammer H, Bornemann S and Macheroux P
Biochemistry 46, 3768-3774 (2007)

A germin-like protein with superoxide dismutase activity in pea nodules with high protein sequence identity to a putative rhicadhesin receptor
Gucciardo S, Wisniewski JP, Brewin NJ and Bornemann S
J Exp Bot 58, 1161-1171 (2007)

Real-time monitoring of the oxalate decarboxylase reaction and probing hydron exchange in the product, formate, using Fourier transform infrared spectroscopy
Muthusamy M, Burrell MR, Thorneley RNF and Bornemann S
Biochemistry 45, 10667-10673 (2006)

Cloning and sequencing of two Ceriporiopsis subvermispora bicupin oxalate oxidase allelic isoforms: implications for the reaction specificity of oxalate oxidases and decarboxylases
Escutia MR, Bowater L, Edwards A, Bottrill AR, Burrell MR, Polanco R, Vicuña R and Bornemann S
Appl Environ Microbiol 71, 3608-3616 (2005)

A closed conformation of Bacillus subtilis oxalate decarboxylase OxdC provides evidence for the true identity of the active site
Just VJ, Stevenson CE, Bowater L, Tanner A, Lawson DM and Bornemann S
J Biol Chem 279, 19867-19874 (2004)

Bacillus subtilis
YxaG is a novel Fe-containing quercetin 2,3-dioxygenase
Bowater L, Fairhurst SA, Just VJ and Bornemann S
FEBS Lett 557, 45-48 (2004)

A branch point in chorismate synthase research
Bornemann S, Lawson DM, and Thorneley RNF
Structure 11, 1463-1465 (2003)

Three isoforms of isoamylase contribute different catalytic properties for the debranching of potato glucans
Hussain H, Mant A, Seale R, Zeeman S, Hinchliffe E, Edwards A, Hylton C, Bornemann S, Smith AM, Martin C and Bustos R
Plant Cell 15, 133-149 (2003)

Flavoenzymes that catalyse reactions with no net redox change
Bornemann S
Nat Prod Rep 19, 761-772 (2002)

Oxalate decarboxylase requires manganese and dioxygen for activity: Overexpression and characterisation of Bacillus subtilis YvrK and YoaN
Tanner A, Bowater L, Fairhurst SA and Bornemann S
J Biol Chem 276, 43627-43634 (2001)

Bacillus subtilis
YvrK is an acid-induced oxalate decarboxylase
Tanner A and Bornemann S
J Bact 18, 5271-5273 (2000)

Studies with substrate and cofactor analogues provide evidence for a radical mechanism in the chorismate synthase reaction
Osborne A, Thorneley RNF, Abell C and Bornemann S
J Biol Chem 275, 35825-35830 (2000)

A secondary beta deuterium kinetic isotope effect in the chorismate synthase reaction
Bornemann S, Theoclitou ME, Brune M, Webb MR, Thorneley RNF and Abell C
Bioorg Chem 28, 191-204 (2000)

Barley (Hordeum vulgare) oxalate oxidase is a manganese-containing enzyme
Requena L and Bornemann S
Biochem J 343, 185-190 (1999)

Specificity of starch synthase isoforms from potato
Edwards A, Borthakur A, Bornemann S, Venail J, Denyer K, Waite D, Fulton D, Smith A and Martin C
Eur J Biochem 266, 724-736 (1999)