We sequenced the 723,970-bp AY-WB genome to completion. Phytoplasmas cannot be cultured, and therefore the AY-WB genome was isolated from pulse field gels. A shotgun library was prepared and the library was sequenced to saturation. Sequences were checked for quality and assembled into five contigs. These contigs represent the 706,569 bp chromosome, and plasmids pAYWB-I (3,972 bp), pAYWB-II (4,009 bp), pAYWB-III (5,104 bp), and pAYWB-IV (4,316 bp)

The chromosome contains 671 ORFs, and the plasmids a total of 22 ORFs. Sequences and annotation can be downloaded from GenBank:

Aster yellows witches'-broom phytoplasma AYWB, complete genome DNA; circular; Length: 706,569 nt; Replicon Type: chromosome; Accession number: NC_007716

Aster yellows witches'-broom phytoplasma AYWB plasmid pAYWB-I, complete sequence DNA; circular; Length: 3,972 nt; Replicon Type: plasmid; Accession number: NC_007717

Aster yellows witches'-broom phytoplasma AYWB plasmid pAYWB-II, complete sequence DNA; circular; Length: 4,009 nt; Replicon Type: plasmid; Accession number: NC_007718

Aster yellows witches'-broom phytoplasma AYWB plasmid pAYWB-III, complete sequence DNA; circular; Length: 5,104 nt; Replicon Type: plasmid; Accession number: NC_007719

Aster yellows witches'-broom phytoplasma AYWB plasmid pAYWB-IV, complete sequence DNA; circular; Length: 4,316 nt; Replicon Type: plasmid; Accession number: NC_007720

ACKNOWLEDGEMENT

If you make use of this data, please cite the following manuscript:

Bai X., Zhang J., Ewing E., Miller S. A., Radek A., Schevchenko D., Tsukerman K., Walunas T., Lapidus A., Campbell, J. W. and Hogenhout S. A. (2006). Living with genome instability: the adaptation of phytoplasmas to diverse environments of their insect and plant hosts. J. Bact. 188: 3682-3696. Hogenhout2006.pdf