Plant Transformation Vectors

Vera Thole & Philippe Vain

         

pCLEAN dual binary vector system

 

Added features of pCLEAN vectors:

(compared to previous pGreen and pSoup-based vectors)

• • Counter selection of backbone transfer
• • Modified left border (LB) of T-DNA
• • Minimal T-DNA (only 52 nt excluding the borders)
• • Reduced homology between pCLEAN and pSoup
• • Multiple modified LBs
• • Additional vir genes
• • Exploiting backbone transfer for stable transformation
• • Delivering multiple T-DNAs (clean-gene transformation)
   

pBrachyTAG dual binary vector system

Added features of pBrachyTAG vectors:
(compared to previous pGreen and pSoup-based vectors)

• Modified left border (LB) of T-DNA

• Reduced homology between pBrachyTAG and pSoup

• Multiple modified LBs

• Additional vir genes

• UniBox sequence to facilitate the retrieval of FSTs

• Promoter trapping features

The pCLEAN, pBrachyTAG and pGreen/pSoup dual binary vector systems are fully compatible enabling pGreen-based and pSoup-based vectors to be mixed and matched in Agrobacterium.