pCLEAN

Philippe Vain – John Innes Centre

 

pCLEAN vectors (Thole et al., 2007) are compatible with the dual binary vector system pGreen and pSoup, where two binary vectors (i.e. pGreen and pSoup) are both present in the same Agrobacterium.

 

In Agrobacterium, pSoup provides replication function in trans for pGreen (Hellens et al., 2000). Both pGreen and pSoup can be maintained individually in Escherichia coli.

 

Information on basic pGreen and pSoup vectors can be found

http://www.pgreen.ac.uk/a_pls_fr.htm

 

There are two families of pCLEAN vectors:

 

•   pCLEAN-G (which can be used as a pGreen equivalent)

•   pCLEAN-S (which can be used as a pSoup equivalent)

 

pCLEAN, pGreen and pSoup vectors can be mixed and matched in

Agrobacterium:

Added features in pCLEAN vectors:

(compared to other pGreen- and pSoup-based vectors)

 

•   Counter selection of backbone transfer

•   Modified left border (LB) of T-DNA

•   Minimal T-DNA (only 52 nt excluding the borders)

•   Reduced homology between pCLEAN and pSoup

•   Multiple modified LBs

•   Additional vir genes

•   Exploiting backbone transfer for stable transformation

•   Delivering multiple T-DNAs (clean-gene transformation)

 

 

Summary of GenBank accession numbers:

pCLEAN-G vectors:                                       pCLEAN-S vectors:

EU186081     pCLEAN-G121                         EU186085 pCLEAN-S161

EU186082     pCLEAN-G126                         EU186086 pCLEAN-S167

EU186090     pCLEAN-G129

EU186091     pCLEAN-G130

EU186089     pCLEAN-G144

EU186088     pCLEAN-G146

EU186087     pCLEAN-G147

EU186083     pCLEAN-G181

EU186092     pCLEAN-G182

EU186084     pCLEAN-G185

 

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