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Abstract:
The multiparent advanced intercross (MAIC) offers a compromise
between linkage mapping and LD mapping: QTL can be located to
smaller intervals than the former without the increase in false
positives of the latter. Moreover, the MAIC can be used to
derive material suitable for coarse mapping from early
generations and for fine mapping from later generations. It also
has a high probability of segregating for multiple QTL. This
proposal is very timely because the MAIC takes time to
construct. By establishing MAIC populations now, they will
provide material in the future for fine mapping with high
density genotyping. The development of high density marker sets
is an area of active research. There is a risk that such
research will be successful but have no application because
suitable populations for fine mapping do not exist. By setting
up MAIC populations now, they will be well placed to exploit
high throughput methods as they become available in the fairly
near future. We will establish two MAIC populations in winter
wheat and derive 1000 inbred lines from each. One will have 8
founders selected from contemporary elite UK cultivars, the
other 16 founders of historic importance. At least 1 founder
will be common to both populations. The combination of elite and
broadly based populations will establish a resource appropriate
for fine mapping multiple QTL for multiple traits. We have full
access to two broadly based populations of winter wheat,
segregating for genetic male sterility (gms). These populations
have been maintained through outcrossing. Although subject to
some selection, they have been maintained in isolation and are
suitable for fine mapping. From each, 1000 inbred lines will be
derived for mapping studies. DArT genotyping on the founder
lines, samples of the inbred lines and outcrossed individuals
will be used to test the power and precision of all the
populations for fine mapping. The data will also be used to fine
map the gms loci.
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