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The Moore Lab

Understanding the make-up of the Ph1 complex and its role in chromosome pairing

Selected publications

Ph1- molecular characterisation.

Al-Kaff N, Knight E, Bertin I, Foote T, Hart N, Griffiths S, & Moore G (2008).  Detailed dissection of the chromosomal region containing the Ph1 locus in wheat Triticum aestivum: With deletion mutants and expression profilingAnn. Bot. 101: 863-872 doi:10.1093/aob/mcm252

Griffiths S, Sharp R, Foote TN, Bertin I, Wanous M, Reader S, Colas I, Moore G (2006) Molecular characterization of Ph1 as a major chromosome pairing locus in polyploid wheat. Nature 439: 749-752.  Figure legends to accompany supplementary information  (Cell Research Highlight- Leading edge- Cell (2006) 124: 869)

Roberts M, Reader S, Dalgliesh C, Miller T, Foote T, Fish L, Snape J, Moore G (1999) Induction and characterisation of Ph1 wheat mutants. Genetics 153: 1909-1918.

Qu L-J, Foote T, Roberts M,  Money T, Aragon-Alcaide L, Snape J, Moore G (1998) A simple PCR-based method for scoring the ph1b deletion in wheat. Theor. Appl. Genet. 96: 371-375.

Foote T, Roberts M, Kurata N, Sasaki T, Moore G (1997) Detailed comparative mapping of cereal chromosome regions corresponding to the Ph1 locus in wheat.  Genetics 147: 801-807.

Clarke B, Stancombe P, Money T, Foote T, Moore G (1992) Targeting deletion (homoeologous chromosome pairing locus) or addition line single copy sequences from cereal genomes. Nucleic Acids Res. 20: 1289-1292.

Ph1- its cell biological effect.

Moore G and Shaw P (2009) Improving the chances of finding the right partner. Curr Opin Genet Dev.  In Press

Colas I, Shaw P, Prieto P, Wanous M, Speilmeyer W, Mago R, Moore G (2008)
Effective chromosome pairing requires chromatin remodelling at the onset of meiosis. Proc. Natl. Acad. USA  105: 6075-6080; published online before print April 15, 2008, doi:10.1073/pnas.0801521105

Prieto P, Shaw P, Moore G (2004) Homologue recognition during meiosis is associated with a change in chromatin conformation.  Nature Cell. Biol. 6: 906-908.

Cell biology of wheat meiosis

Martinez-Perez E, Shaw P, Reader S, Aragon-Alcaide L, Miller T, Moore G (1999) Homologous chromosome pairing in wheat. J. Cell Sci. 112: 1761-1769.

Aragon-Alcaide L, Reader S, Beven A, Shaw P, Miller T, Moore G (1997) Association of homologous chromosomes during floral development. Curr. Biol. 7: 905-908.

Centromeres

Martinez-Perez E, Shaw P, Aragon-Alcaide L, Moore G (2003) Chromosomes form into seven groups in hexaploid and tetraploid wheat as a prelude to meiosis. Plant J 36: 21-39.

Martinez-Perez E, ShawP, Moore G (2001) The Ph1 locus is needed to ensure specific somatic and meiotic centromere association. Nature 411: 204-207. Front cover Nature 411 (Weeding out improper centromere pairs).

Martinez-Perez E, Shaw P, Moore G (2000) Polyploidy induces centromere association. J. Cell Biol.148: 233-238.

Aragon-Alcaide L, Miller T, Schwarzacher T, Reader S, Moore G. (1996) A cereal centromeric sequence. Chromosoma 105: 261-268.

Comparative genomics

Moore G, Devos K, Wang Z, Gale M (1995) Cereal genome evolution: Grasses, line up and form a circle. Curr. Biol.  5: 737-739.

Dunford, R, Kurata N, Laurie D, Money T, Minobe Y, Moore G (1995) Conservation of fine-scale DNA marker order in the genomes of rice and the Triticeae. Nucleic Acids Res. 23: 2724-2728.

Kurata N, Moore G, Nagamura Y, Foote T, Yano M, Minobe Y, Gale M (1994) Conservation of genome structure between rice and wheat. Nature Biotech.  12: 276-278.

Moore G, Gale MD, Kurata N, Flavell R.(1993) Molecular analysis of small grain cereal genomes. Nature Biotech. 11: 584-589.

Resources and Technologies

Foote T, Griffiths S, Allouis S, Moore G (2004)  Construction and analysis of a BAC library in the grass Brachypodium sylvaticum: its use as a tool to bridge the gap between rice and wheat in elucidating gene content.  Funct. Integr. Genomics 4: 26-33. BBSRC BAC library available free of any IP from the John Innes Genome Laboratory

Allouis S, Moore G, Bellec A, Sharp R, Faivre-Rampant P, Mortimer K, Pateyron S, Foote T, Griffiths S, Caboche M, Chalhoub B. (2003) Construction and characterisation of a hexaploid wheat (Triticum aestivum  L) BAC library from the reference germplasm “Chinese Spring”. Cereals Res. Commun. 31: 331-338. BBSRC-INRA BAC library available free of any IP from the John Innes Genome Laboratory

Money T, Reader S, L-J Qu, Dunford R, Moore G (1996)  AFLP-based mRNA fingerprinting. Nucleic Acids Res.  24: 2616-2617.

Wang M, Leitch A, Schwarzacher T, Heslop-Harrison JS, Moore G. (1992) Construction of a chromosome-enriched HpaII library from flow-sorted wheat chromosomes. Nucleic Acids Res. 20: 1897-1901.

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