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Full Publication List and Patents: Richard Hughes

Refereed Papers

  1. Domenico, S., Tsesmetzis, N., Di Sansebastiano, G. P., Hughes, R. K., Casey, R. and Santino, A. (2007) Subcellular localisation of Medicago truncatula9/13 hydroperoxide lyase reveals a new localisation pattern and activation mechanism for CYP74C enzymes. BMC Plant Biology 7: 58
  2. Belfield, E. J., Hughes, R. K., Tsesmetzis, N., Naldrett, M. J. and Casey, R. (2007) The Gateway pDEST17 expression vector encodes a –1 ribosomal frameshifting sequence. Nucleic Acids Research 35, 1322-1332
  3. Hughes, R. K., Belfield, E. J., Casey, R. (2006) CYP74C3 and CYP74A1, plant cytochrome P450 enzymes whose activity is regulated by detergent micelle-association and proposed new rules for the classification of CYP74 enzymes. Biochemical Society Transactions 34, 1223-1227
  4. Hughes, R. K., Belfield, E. J., Ashton, R., Fairhurst, S. A., Göbel, C., Feussner, I. and Casey, R. (2006) Allene oxide synthase from Arabidopsis thaliana (CYP74A1) exhibits dual specificity that is regulated by monomer-micelle association. FEBS Letters 580, 4189-4195
  5. Hughes, R. K., Belfield, E. J., Muthusamay, M., Khan, A., Rowe, A., Harding, S. E., Fairhurst, S. A., Bornemann, S., Ashton, R., Thorneley, R. N. F. and Casey, R. (2006) Characterisation of Medicago truncatula hydroperoxide lyase, a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer-micelle association. Biochemical Journal 395, 641-652
  6. Khan, A., Hughes, R. K., Belfield, E. J., Casey, R., Rowe, A. J. and Harding, S. E. (2006) Oligomerization of hydroperoxide lyase, a novel P450 enzyme in plants. Progress in Colloid and Polymer Science 131, 116-120
  7. Mita, G., Quarta, A., Fasano, P., Paolis, A. D., Sansebastiano, G. P. D., Perrotta, C., Iannacone, R., Belfield, E., Hughes, R., Casey, R. and Santino, A. (2005) Molecular cloning and characterisation of an almond 9-hydroperoxide lyase, a new CYP74 targeted to lipid bodies. Journal of Experimental Botany 56, 2321-2333 + Front Cover
  8. Durand, A., Hughes, R., Roussel, A., Flatman, R., Henrissat, B. and Juge, N. (2005) Emergence of a subfamily of xylanase inhibitors within glycoside hydrolase family 18. FEBS Journal 272, 1745-1755 [See also FEBS Journal 272, 3227]
  9. Santino, A. Iannacone, R., Hughes, R. Casey, R. and Mita, G. (2004) Cloning and characterisation of an almond 9-lipoxygenase expressed early during seed development. Plant Science 168, 699-706
  10. Elliott, G. O., Hughes, R. K., Juge, N., Kroon, P. A. and Williamson, G. (2002) Functional identification of the cDNA coding for a wheat endo-1,4-ß-D-xylanase inhibitor. FEBS Letters 519, 66-70
  11. Flatman, R., Mclauchlan, W. R., Juge, N., Furniss, C. S. M., Berrin, J-G., Hughes, R. K., Manzanares, P., Ladbury, J. and Williamson, G. (2002) Interactions defining the specificity between fungal xylanases and the wheat proteinaceous inhibitor, XIP-I. Biochemical Journal 365, 773-781
  12. Hughes, R. K., Desforges, N., Selwood, C., Speirs, C. I., Sinnaeve, G., Gorton, P. G., Wiseman, J., Jumel, K., Harding, S. E., Hill, S. E., Street, V., Wang, T. L. and Hedley, C. L. (2001) Genes affecting starch biosynthesis exert pleiotropic effects on the protein content and composition of pea seeds. Journal of the Science of Food and Agriculture 81, 877-882
  13. Hughes, R. K., Lawson, D. M., Hornostaj, A. R., Fairhurst, S. A. and Casey, R. (2001) Mutagenesis and modelling of linoleate-binding to pea seed lipoxygenase. European Journal of Biochemistry 268, 1030-1040
  14. Hughes, R. K., Hornostaj, A. R., Lawson, D. M., West, S. I., Fairhurst, S. A., Sanchez, R. O., Hough, P., Robinson, B. H. and Casey, R. (2001) Probing a novel potato lipoxygenase with dual positional specificity reveals primary determinants of substrate binding and requirements for a surface hydrophobic loop and has implications for the role of lipoxygenases in tubers. Biochemical Journal 353, 345-355
  15. Wu, Z., Robinson, D. S., Hughes, R. K., Casey, R., Hardy, D. and West, S. I. (1999) Cooxidation of β-carotene catalyzed by soybean and recombinant pea lipoxygenases. Journal of Agricultural and Food Chemistry 47, 4899-4906
  16. Busto, M. D., Owusu-Apenten, R. K., Robinson, D. S., Wu, Z., R. Casey, Hughes, R. K. (1999) Kinetics of thermal inactivation of pea seed lipoxygenases and the effect of additives on their thermostability. Food Chemistry 65, 323-329
  17. Hughes, R. K., Wu, Z., Robinson, D.S., Hardy, D., West, S. I., Fairhurst, S. A. and Casey, R. (1998) Characterisation of authentic recombinant pea seed lipoxygenases with distinct properties and reaction mechanisms. Biochemical Journal 333, 33-43
  18. Hughes, R. K., Perbal, M. C., Maule, A.J. and Hull, R. (1995) Evidence for proteolytic processing of tobacco mosaic virus movement protein in Arabidopsis thaliana. Molecular Plant-Microbe Interactions 8, 658-665
  19. Hughes, R. K. (1992) Xanthine dehydrogenase from Drosophila melanogaster: purification and properties of the wild-type enzyme and of a variant lacking iron-sulphur centres. Biochemistry 31, 3073-3083
  20. Hughes, R. K., Doyle, W.A., Chovnick, A., Whittle, J.R.S., Burke, J.F. and Bray, R.C. (1992) Use of rosy mutant strains of Drosophila melanogaster to probe the structure and function of xanthine dehydrogenase. Biochemical Journal 285, 507-513 [See also Biochemical Journal 300, 917 (1994)]
  21. Hughes, R. K., Bennett, B., Doyle, W.A., Burke, J.F., Chovnick, A., and Bray, R.C. (1991) Roles of molybdenum, FAD and iron-sulphur domains in molybdenum-containing hydroxylases: molecular genetic, kinetic and spectroscopic studies. Biochemical Society Transactions 19, 260S
  22. Hughes, R. K. and Dickerson, A.G. (1991) Modulation of elicitor-induced chitinase and β-1,3-glucanase activity by hormones in Phaseolus vulgaris. Plant and Cell Physiology 32, 853-861
  23. Hughes, R. K. and Dickerson, A.G. (1990) Auxin regulation of the response of Phaseolus vulgaris to a fungal elicitor. Plant and Cell Physiology 31, 667-675
  24. Hughes, R. K. and Dickerson, A.G. (1989) The effect of ethylene on phenylalanine ammonia-lyase (PAL) induction by a fungal elicitor in Phaseolus vulgaris. Physiological and Molecular Plant Pathology 34, 361-378

Review Articles

  1. Casey, R. and Hughes, R. K. (2004) Recombinant lipoxygenases and oxylipin metabolism in relation to food quality. Food Biotechnology 18, 135-170
  2. Casey, R., West, S. I., Hardy, D., Robinson, D. S., Wu, Z. and Hughes, R. K. (2000) New frontiers in food enzymology: recombinant lipoxygenases. Trends in Food Science and Technology 10, 297-302
  3. Hughes, R. K., Wu, Z., Hornostaj, A. R., Robinson, D. S., Hardy, D., West, S. I., Owusu-Apenten, R. K., Fairhurst, S. A., Chan, M., Lawson, D. M. and Casey, R. (1999) Recombinant pea and potato lipoxygenases: versatile biocatalysts for industry. AgroFoodIndustry Hi-Tech 10, 3-6.
  4. Hughes, R. K., West, S. I. and Casey, R. (1999) Recombinant plant lipoxygenases. AgBiotechNet 1, October, pp. 1-4, CAB International.

Conference Proceedings

  1. Hughes, R. K., Belfield, E. J., Muthusamay, M., Khan, A., Rowe, A., Harding, S. E., Fairhurst, S. A., Bornemann, S., Ashton, R., Thorneley, R. N. F. and Casey, R. (2006) CYP74C3 and CYP74A2: plant cytochrome P450 enzymes whose activity is regulated by detergent micelle-association Proceedings of 8th International Symposium on P450 Biodiversity and Biotechnology, 23 - 27 July, Medical School, University of Swansea, Wales, U.K.
  2. Belfield, E., Chechetkin, I., Hughes, R., Tsesmetzis, N. and Casey, R. (2006) Properties of hydroperoxide lyases (HPLs) from Medicago truncatula. Abstract of 2nd International Conference on Nonmammalian Eicosanoids, Bioactive Lipids and Plant Oxylipins, May, Berlin, Germany, p. 62.
  3. Belfield, E., Hughes, R., Tsesmetzis, N. and Casey, R. (2006) Manipulation of the enzymes of plant oxylipin metabolism. In Proceedings of SEB meeting: Comparative Biochemistry and Physiology, Part A 143: S22 (P6.5), 2-7 April, University of Kent at Canterbury U.K.
  4. Belfield, E., Hughes, R., Olias, R., Tsesmetzis, N. and Casey, R. (2005) Characterisation and expression of lipoxygenases and hydroperoxide lyases. In Proceedings of GARNet meeting: 2010 and Beyond, 5-6 September, John Innes Centre, Norwich, U.K., p. 39.
  5. Khan, A. Harding, S. E., Belfield, E. J., Casey, R. and Hughes, R. K. (2005) Oligomerization of hydroperoxide lyase, a novel P450 enzyme in plants. 14th International Symposium on Analytical Ultracentrifugation (AUC 2005), 3-4 March, EPFL-Lausanne, Switzerland.
  6. Casey, R., Belfield, E., Hughes, R., Olias, R. and Tsesmetzis, N. (2004) Lipoxygenases, Oxylipins and flavour production. Proceedings of 228th ACS National Meeting, paper 784635, August 22-26, Philadelphia, USA.
  7. Belfield, E., Hughes, R., Olias, R., Tsesmetzis, N. and Casey, R. (2004) Characterisation and expression of lipoxygenases and hydroperoxide lyases. In Proceedings of the 9th International Symposium on Plant Seeds: “Seeds in the –omics era”, Gatersleben, Germany, p. 87.
  8. Belfield, E., Hughes, R. K., Olias, R., Tsesmetzis, N. and Casey, R. (2004) Expression and characterisation of legume lipoxygenases and hydroperoxide lyases. Proceedings of Society for Experimental Biology meeting, 29 March-2 April, Herriot-Watt University, Edinburgh, U.K.
  9. Olias, R., Belfield, E., Hughes, R., and Casey, R. (2003) Characterisation and expression of lipoxygenases and hydroperoxide lyases. Proceedings of Biochemical Society Meeting, June 12-14, University of Aachen, Germany.
  10. Hughes, R., Belfield, E., Lawson, D., Stevenson, C., Durrant, M. and Casey, R. (2003) Towards the structure of the first plant cytochrome P450, hydroperoxide lyase from Medicago truncatula: a structural approach to understand oxylipin metabolism in plants. Proceedings of 15th John Innes Symposium: Structural Challenges in the Post-Genomic Era, 15-18 July, Norwich, U.K.
  11. Iannacone, R., Mita, G., Gallo, A., Hughes, R., Casey, R., Perito, A., Santino, A. and Cellini, F. (2003) An almond 9-lipoxygenase expressed in transformed tobacco plants. Proceedings of 7th International Congress of Plant Molecular Biology, June 16-21, Session S01 - Lipid Metabolism, Reference S01-9, Barcelona, Spain.
  12. Belfield, E., Hughes, R., Olias, R. and Casey, R. (2003) Characterisation and expression of lipoxygenases and hydroperoxide lyases. Proceedings of 14th International Conference on Arabidospis Research, June 12-14, University of Wisconsin, USA.
  13. Hughes, R., Belfield, E., Olias, R. and Casey, R. (2003) Characterisation and expression of lipoxygenases and hydroperoxide lyases. Proceedings of Plant Oxylipins conference: 2nd Joint Symposium of the EU-Consortium on Natural Oxylipins and Defence in Ornamentals (NODO), Working Group on Non-Mammalian Eicosanoids and the German Society for Fat Science (DGF), June 12-14, University of Göttingen, Germany.
  14. Mita, G., Quarta, A., De Paolis, A., Di Sansebastiano, G.P., Fasano, P., Casey, R., Hughes, R., Perrotta, C., Santino, A. (2003) Molecular cloning and characterisation of a putative hydroperoxide lyase gene from almond. Proceedings of Plant Oxylipins conference: 2nd Joint Symposium of the EU-Consortium on Natural Oxylipins and Defence in Ornamentals (NODO), Working Group on Non-Mammalian Eicosanoids and the German Society for Fat Science (DGF), June 12-14, University of Göttingen, Germany.
  15. Hughes, R., Belfield, E., Olias, R. and Casey, R. (2003) Characterisation and expression of lipoxygenases and hydroperoxide lyases. Proceedings of Biochemical Society Focused Meeting: Enzyme Mechanism - A Structural Perspective, 12-14 January, St. Andrews University, Scotland, U.K.
  16. Juge, N., Flatman, R., Tahir, T., Furniss, C.S.M., Elliot, G.O., Hughes, R., Roussel, A., Payan, F., McLauchlan, W.R., Kroon, P.A. and Williamson, G. (2002) Study of XIP-I, a xylanase inhibitor protein from wheat, and its interaction with xylanases. Proceedings of the Third European Symposium on Enzymes in Grain Processing (Courtin, C.M., Veraverbeke, W.S. and Delcour, J.A., Eds.), September 25-27, Katholieke Universiteit Leuven, Leuven, Belgium.
  17. Olias, R., Belfield, E., Hughes, R. K. and Casey, R. (2002) Molecular evolution of functionally improved lipoxygenases. Proceedings of 54th Harden Conference: Enzymology - Emerging Trends and Future Prospects, 20-24 August, St. Martin's College, Ambleside, Lake District, U.K.
  18. Olias, R., Belfield, E., Hughes, R. K. and Casey, R. (2002) Molecular evolution of functionally improved lipoxygenases for commercial exploitation. Proceedings of Biotechnology meeting, Seville, Spain.
  19. Olias, R., Belfield, E., Hughes, R. K. and Casey, R. (2002) Molecular evolution of functionally improved lipoxygenases. Proceedings of 13th International Conference on Arabidopsis Research, June 28-July 2, Seville, Spain.
  20. Hughes, R. K., Roussell, A., Payan, F., Williamson, G. and Juge, N. (2002) Cloning and secretion of a wheat endo-1,4-ß-D-xylanase inhibitor (XIP-I) and related proteins in Pichia pastoris to probe their functionality as members of family 18 glycosyl hydrolases Proceedings of Workshop on Proteinaceous Inhibitors of Glycoside and Protein Hydrolases, Marseilles, France.
  21. Juge, N., Flatman, R., Tahir, T., Furniss, C. S. M., Elliot, G. O., Sancho, A., Hughes, R., McLauchlan, W. R., Kroon, P. A. and Williamson, G. (2002) Xylanase inhibitor protein (XIP-I) from wheat. Proceedings of Workshop on Proteinaceous Inhibitors of Glycoside and Protein Hydrolases, Marseilles, France.
  22. Wang, T. L., Johnson, S., Harrison, C, Hughes, R. and Hedley, C. L. (2002) The relationship between storage product accumulation and seed development in legumes. 1st International Conference on Legume Genomics and Genetics: Translation to Crop Improvement, Plenary Session VIII: Reproductive Biology, Minneapolis, St. Paul, MN., USA.
  23. Hughes, R. K., Hornostaj, A. R., Robinson, D. S., Casey, R., Wang, T. L. and Hedley, C. L. (2000) Genetic manipulation of starch biosynthesis in pea seeds induces changes in lipoxygenase activity and has implications for the agrifood industry. Proceedings of International Conference on Nutritional Enhancement of Plant Derived Foods in Europe (NEODIET), Norwich, U.K.
  24. Casey, R., Hughes, R. K., West, S. I., Hardy, D., Robinson, D. S., Wu, Z. (1999) Recombinant lipoxygenases for the improvement of dough quality in breadmaking. Proceedings of Society for Industrial Microbiology meeting: 3rd Conference on Recent Advances in Fermentation Technology (RAFT III), Sarasota, Florida USA, S31, p.30.
  25. Hughes, R. K., Forster, C., Casey, R. West, S. I., Hardy, D., Hornostaj, A. R., Robinson, D. S., Wu, Z. and North, H. M. (1999) Pea lipoxygenases: authentication of recombinant enzymes and removal from seeds confirms they are functionally distinct and has implications for the agrifood industry. Proceedings of the 23rd World Congress and Exhibition of the International Society for Fat Research, Royal Society of Chemical Industry, Brighton, U.K., p. 70.
  26. Casey, R., Domoney, C., Hornostaj, A., Hughes, R., Forster, C., North, H., Robinson, D. S. and Wu, Z. (1998) Fatty acid oxidation, tasteful tunes played on the green notes. Proceedings of Biochemical Society Meeting, Leicester.
  27. Hughes, R. K., Wu, Z., Hornostaj, A. R., Robinson, D. S., Hardy, D., West, S. I., Owusu-Apenten, R., Fairhurst, S. A., Chan, M., Lawson, D. M. and Casey, R. (1998) Recombinant pea and potato lipoxygenases for industry. Catalysis Technology “Car Boot Sale”, Royal Society of Chemistry, Manchester, U.K.
  28. Hull, R., Hughes, R. K., Wallis, C.V., Serghini, M.A. and Atkins, D. (1994) Introduction to virus movement in plants and movement of tobamoviruses. BRIDGE Final Sectorial Meeting: Plant-Microbe Interactions, Dourdan, France.
  29. Maule, A. J., Hughes, R., Perbal, M. C. and Thomas, C. L. (1993) Functional analysis of the cauliflower mosaic virus movement protein. Proceedings of the 12th Annual Meeting of the American Society of Virologists, Davies, USA.
  30. Maule, A.J., Perbal, M. C, Hughes, R. and Thomas, C.L. (1993) A functional analysis of the cauliflower mosaic virus movement protein. Proceedings of the IXth International Congress of Virology, Glasgow, Scotland, W63-5, 99.
  31. Bray, R. C., Hughes, R. K., Doyle, W. A., Bennett, B. and Burke, J. F. (1992) The use of rosy xanthine dehydrogenase variants from Drosophila melanogaster in probing the functioning of molybdenum enzymes. Proceedings of SERC Molecular Recognition Initiative Workshop, University of Cambridge.
  32. Hughes, R. K., Bennett, B., Doyle, W. A., Burke, J. F., Chovnick, A. and Bray, R. C. (1991) Roles of molybdenum, FAD and iron-sulphur domains in molybdenum-containing hydroxylases: molecular genetic, kinetic and spectroscopic studies. Proceedings of Biochemical Society Meeting, Reading, 260S, 19.
  33. Bray, R. C., Bennett, B., Burke, J. F., Doyle, W. A. and Hughes, R. K. (1991) Defining the active sites of pterin molybdenum cofactor enzymes from spectroscopic and molecular genetic studies. Biofactors 3, 145.
  34. Bray, R. C., Hughes, R. K., Doyle, W. A., Whittle, J. R. S. and Burke, J. F. (1991) Towards an expression system for site-directed mutagenesis studies of xanthine dehydrogenase (Drosophila melanogaster rosy gene). Proceedings of the 10th International Symposium on Flavins and Flavoproteins, Como, Italy.
  35. Hughes, R. K., Chamberlain, J., Wallis, G. and Dickerson, A.G. (1989) Hormonal regulation of elicitor-induced chitinase and β-1,3-glucanase activity in Phaseolus vulgaris. Proceedings of Society for Experimental Biology Symposium: Hormone Perception and Signal Transduction in Animals and Plants, University of Nottingham, Nottingham, U.K.

Edited Contributions to Books and Conferences

  1. Khan, A., Hughes, R. K., Belfield, E. J., Casey, R., Rowe, A. J. and Harding, S. E. (2006) “Oligomerization of hydroperoxide lyase, anovel P450 enzyme in plants” in: Analytical Ultracentrifugation VIII, (Wandrey, C. and Cölfen, H. eds), pp.115–119, Springer Berlin/Heidelberg, Germany. See the book on Amazon.
  2. Casey, R. C. and Hughes, R. K. (2005) “Recombinant lipoxygenases and oxylipin metabolism in relation to food quality” in: Food Biotechnology, 2nd Edition (Shetty, K., Paliyath, G., Pometto, A., Levin, R.E. eds.), Chapter 2.18, pp. 989-1020. Marcel Dekker. See the book on Amazon.
  3. Casey, R., Belfield, E., Hughes, R., Olias, R. and Tsesmetzis, N. (2004) Lipoxygenases, oxylipins and flavour production. Abstract of papers of the American Chemical Society 228: 045-AGFD Part 1, 22 August, Philadelphia, U.S.A.
  4. Juge, N., Flatman, R., Tahir, T., Furniss, C.S.M., Elliot, G.O., Hughes, R., Roussel, A., Payan, F., McLauchlan, W.R., Kroon, P.A. and Williamson, G. (2003) “Study of XIP-I, a xylanase inhibitor protein from wheat, and its interaction with xylanases” in: Recent Advances in Enzymes in Grain Processing, Proceedings of the Third European Symposium on Enzymes in Grain Processing (Courtin, C.M., Veraverbeke, W.S. and Delcour, J.A., Eds.), p. 179-183, Katholieke Universiteit Leuven, Leuven, Belgium.
  5. Hughes, R. K., Wu, Z., Robinson, D. S., Williams, C. E. M., Lawson, D. M., Fairhurst, S. A. and Casey, R. (1997) Characterization of authentic recombinant pea seed lipoxygenases. Perspectives on Protein Engineering 2 (M. J. Geisow, ed.), BIODIGM (CD-ROM), ISBN 0-9529015-1-X.
  6. Hughes, R. K., Wu, Z., Robinson, D. S. and Casey, R. (1997) “Production of pea seed lipoxygenases in Escherichia coli” in: Plant Proteins from European Crops - Food and Non-Food Applications (J. Gueguen and Y. Popineau, eds., Springer, 94-98.

Popular Articles

  1. Eastern Daily Press article on NODO project (15 December 2003).
  2. Recombinant LOX enzymes for the food and pharmaceutical sectors, Biotechnology and Biological Sciences Research Council (BBSRC) Annual Report 1998-99, 24.
  3. Hughes, R. K., Forster, C. and Casey, R. (1999) The properties of different pea seed lipoxygenases have implications for industrial application and crop quality. John Innes Centre and Sainsbury Laboratory Annual Report (1998/99), 47-48.
  4. Casey, R. and Hughes, R. K. (1998) From food to pharmaceuticals – the multipurpose lipoxygenases. Biotech LINK, Laboratory of the Government Chemist, M. Geisow, Ed.; Issue 4, 4-5.
  5. Lipoxygenases could find many applications. Industrial Bioprocessing October 23, 1998. Technical Insights, John Wiley & Sons Inc., New York, U.S.A.
  6. Maule, A.J., Thomas, C.L., Perbal, M. C, Hughes, R. K., Davies, J.W., Boulton, M.I., Stanley, J., von Arnim, A., Frischmuth, T., Hull, R., Atkins, D., Wallis, C.V. and Othman, Y. (1992) Virus movement in plants. IPSR and JIC Annual Report, 36.

Patents

“Method and Composition for Enzyme Storage” or “Technology for Storage of Hydroperoxide Lyase and other CYP74 Enzymes”: Tech ID 06.408. UK Patent filed, October 2006

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