 Rachel Melton
I have been Anne's Research Assistant since 1994 and have been involved in many different research projects in that time, as well as being responsible for the smooth running of the lab.
Currently I am investigating the regulation of
synthesis of defence-related compounds (avenacins) in oats
by expression of promoter-reporter fusions in heterologous
species as a preliminary to transferral of single and multiple
steps of the pathway into other plant species. I am also developing
LC/MS-based methods for metabolite fingerprinting of our extended
collection of 100 avenacin-deficient mutants of diploid oat.
In addition I am screening tomato transformants for silencing
of an apoplastic enzyme implicated in the sequential processing
of steroidal molecules in plant/fungus interactions.
Funding
Grant in aid to the John Innes Centre from the BBSRC.
Publications
Xiaoquan Qi , Saleha Bakht, Bo Qin, Mike Leggett, Andrew Hemmings , Fred Mellon, John Eagles , Daniele Werck-Reichhart , Hubert Schaller , Agnes Lesot , Rachel Melton, and Anne Osbourn. (2006) A different function for a member of an ancient and highly conserved cytochrome P450 family: From essential sterols to plant defense. PNAS 103: 18848-18853.
Qi X, Bakht S, Leggett M, Maxwell C, Melton R and Osbourn A. (2004) A gene cluster for secondary metabolism in oat – Implications for the evolution of metabolic diversity in plants. PNAS 101: 8233-8238.
Bouarab K, Melton R, Peart J, Baulcombe D and Osbourn A. (2002) A saponin-detoxifying enzyme mediates suppression of plant defences. Nature 418:889-92.
Apoorva Bhatt, Helen M Kieser, Rachel E. Melton and Tobias Kieser. (2002)
Plasmid transfer from Streptomyces to Mycobacterium smegmatis by spontaneous transformation Mol. Micro. 43: 135-146.
Haralampidis K, Bryan G, Qi X, Papadapoulou K, Bakht S, Melton R, Osbourn A. (2001) A new class of oxidosqualene cyclases directs synthesis of antimicrobial phytoprotectants in monocots. PNAS 98: 13431-13436.
Martin-Hernandez, AM, Dufresne, M, Hugouvieux, V., Melton, R. and Osbourn, AE. (2000) The effects of targeted replacement of the tomatinase gene on the interaction of Septoria lycopersici with tomato plants. Mol. Plant-Microbe Interact. 13: 1301-1311.
Bryan GT, Labourdette E, Melton RE, Nicholson P, Daniels MJ and Osbourn AE. (1999) DNA polymorphism and host range in the take-all fungus, Gaeumannomyces graminis. Mycological Research, 103: 319-327.
K.Papadopoulou,R E Melton, M. Leggett, M.J.Daniels, A.E.Osbourn. (1999) Compromised disease resistance in saponin deficient plants. PNAS 96: 12923-12928.
R.E.Melton, Lynda Flegg, James K. M. Brown, Richard P. Oliver, Michael J. Daniels and Anne E. Osbourn. (1998) Heterologous Expression of Septoria lycopersici Tomatinase in Cladosporium fulvum: Effects on Compatatible and Incompatible Interactions with Tomato Seedlings. MPMI 11: 228-236.
Tamara Smokvina, Duncan J. Henderson, Rachel E. Melton, Dirk Brolle, Tobias Kieser, David A Hopwood. (1994) Transposition of IS117, the 2.5kb Streptomyces Coelicolor A3(2) "minicircle": roles of open reading frames and origin of tandem insertions. Mol. Micro. 12: 459-468.
S.B.Snapper, R.E.Melton, S.Mustafa, T.Kieser, W.R.Jacobs. (1990) Isolation and charachterisation of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol. Micro. 4 : 1911-1919.
Duncan J Henderson, Dirk-Franz Brolle, Tobias Kieser, Rachel Melton, D.A.Hopwood. (1990) Transposition of IS117(the streptomyces coelicolor A3(2) minicircle) to and from a cloned target site and into secondary chromosomal sites. Mol. Gen. Genet. 224: 65-71.
R. E. Melton and T. Kieser. (1988) Regeneration of Streptomyces Lividans protoplasts on media containing antibacterial and antifungal agents. TIG 1988 4:325
Scott Snapper, Lazlo Lugosi, Antonia Jekkel, Rachel Melton, Tobias Keiser, Barry Bloom, William Jacobs. (1988) Lysogeny and transformation in mycobacteria: Stable expression of foreign genes.PNAS 85: 6987-6991.
Tobias Kieser, Rachel Melton (1988) Plasmid pIJ699, a multi copy positive-selection vector for Streptomyces. Gene, 65: 83-91.
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