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EMS treatment and germination
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In October 2006, we did
treatments with EMS at a range of concentrations to
check for germination rate. The seeds were incubated for
14 hours in the EMS and then washed ten times before
spread on soil.
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Germination percentages
indicated that the optimal mutation rate/survival was in
the 0.25-0.40% interval.We therefore used the seeds from
the 0.25%, 0.30% and 0.40% for the M1 population.
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M1 population
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EMS level |
# seed-producing plants |
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0.25% |
1,571 |
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0.30% |
3,464 |
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0.40% |
1,564 |
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Total
6,599 M1
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M1
growth and harvesting
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Plants were grown
individually in 1-liter pots over the winter of
2006/2007 and bagged by JIC horticultural staff.
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Threshing was a big job,
skillfully and efficiently carried out by Lucy Hicks,
Tilly Eldridge and Pauline during summer 2007.
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Norwich Research Park,
Colney, Norwich NR4 7UH, UK
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