This system especially powerful for the separation of tryptic peptidesis by Reversed-phase HPLC:

a sample is injected from the autosampler and loaded at high-flow rate (using the auxillary pump - 0.1% formic acid / 2% acetonitrile) onto a C18 reverse-phase Trap column (0.3mm ID. x 1mm). A binary gradient is then developed and peptides eluted with increasing organic phase (acetonitrile) from the Trap column, refocussed on a C18 reverse-phase capillary column (0.075mm ID. x 50mm) and directly into the Z-Spray ion-source of the Q-ToF II mass spectrometer.

Using this configuration we have identified up to 250 different tryptic peptides from a single HPLC run.