Metabolite Services
at JIC

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• The Team
• Facilities
  • Agilent LC1100 LC-MS
  • HPLC services
  • Thermo Finnigan Ion-trap
  • Agilent 6890N GC-MS
  • Waters Xevo TQ-S
  • Others
• Topics
  • Measuring metabolites
    • General methodology
    • Sample preparation
    • Spectrophotometric methods
    • HPLC
    • LC-MS
    • GC-MS
  • How LC-MS works
    • Why use LC-MS (and what is it anyway)
    • Ionization
      • Electrospray
      • Atmospheric pressure Chemical Ionization
    • Getting a vacuum
    • Mass measurement
      • Single Quadrupole mass filter
      • Ion Trap
    • Collision-induced Fragmentation
    • How does LC-MS differ from GC-MS?
  • Basic mass-spec interpretation
    • Base masses that you can measure
    • Accurate mass
    • Isotopes
  • Compartmentation
    • Mathematics of gradient separations
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