John Innes Centre

Dr Stephen Bornemann

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Curriculum Vitae

  • 1983-1986 BSc Biochemistry, Kings College London
  • 1986-1987 MSc Applied Molecular Biology and Biotechnology, University College London
  • 1987-1989 Tate & Lyle Research and Technology
  • 1989-1992 PhD in Biological Chemistry with Prof David HG Crout, University of Warwick
  • 1993-1995 Post-doctoral Fellow, University of Sussex
  • 1996-present Project Leader, John Innes Centre
  • 2000-present Chartered Chemist and Member of the Royal Society of Chemistry
  • 2000-present Honorary Senior Lecturer, School of Chemical Sciences and Pharmacy, University of East Anglia
  • 2005-present Associate Head of Biological Chemistry Department
  • 2005-present Chartered Scientist

Stephen Bornemann

Associate Head of Department

Biological Chemistry

Contact details

Tel: +44 (0)1603 450741
stephen.bornemann@bbsrc.ac.uk

Research interests

Our current lines of enquiry include starch and polyglucan biosynthesis in plants and microbes, and symbiosis signalling in plants. We are a multidisciplinary group that studies enzymes at the atomic level using biochemical and biophysical methods along with complementary molecular biology approaches to address physiological function. Enzymes that have been studied previously include cupins, such as oxalate-degading enzymes, and chorismate synthase.

Selected Publications

Kalscheuer K., Syson K., Veeraraghavan U., Weinrick B., Biermann K. E., Liu Z., Sacchettini J. C., Besra G., Bornemann S., Jacobs W. R. (2010)
Self-poisoning of Mycobacterium tuberculosis by targeting GlgE in an alpha-glucan pathway
Nature Chemical Biology 6 (5) 376-384
DOI:doi:10.1038/nchembio.340
Clé C., Gunning A. P., Syson K., Bowater L., Field R. A., Bornemann S. (2008)
Detection of transglucosidase-catalyzed polysaccharide synthesis on a surface in real time using surface plasmon resonance spectroscopy.
Journal of the American Chemical Society 130 (46) 15234-5
DOI:10.1021/ja805264w
Burrell M. R., Just V. J., Bowater L., Fairhurst S. A., Requena L., Lawson D. M., Bornemann S. (2007)
Oxalate decarboxylase and oxalate oxidase activities can be interchanged with a specificity switch of up to 282 000 by mutating an active site lid
Biochemistry 46 12327-12336
Just V. J., Burrell M. R., Bowater L., McRobbie I., Stevenson C. E. M., Lawson D. M., Bornemann S. (2007)
The identity of the active site of oxalate decarboxylase and the importance of the stability of active site lid conformations
Biochemical Journal 407 397-406

Recent Publications

Clé C., Martin C., Field R. A., Kuzmic P., Bornemann S. (2010)
Detection of enzyme-catalyzed polysaccharide synthesis on surfaces
Biocatalysis and Biotransformation 28 (1) 64-71
Tabares L. C., Gatjens J., Hureau C., Burrell M. R., Bowater L., Pecoraro V. L., Bornemann S., Un S. (2009)
pH-dependent structures of the manganese binding sites in oxalate decarboxylase as revealed by high-field electron paramagnetic resonance
Journal of Physical Chemistry B 113 (26) 9016-9025
DOI:10.1021/jp9021807
Rauch G., Ehammer H., Bornemann S., Macheroux P. (2008)
Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity
FEBS Journal 275 1464-1473