The data from MALDI-ToF peptide mass fingerprinting is provided as a simple list of masses (peaklist), usually in the form of an excel spreadsheet.
Database searches are performed using a tool called MASCOT. JIC maintains an internal licenced copy of this software and regularly updated
protein databases. See below for some useful links:
i. Computational Biology Group (JIC only).
ii. The dates of most recent Database Updates
iii. The JIC MASCOT tool search page
The default database for searching will be SPTrEMBL (UniProt), unless specified
otherwise. Proteins are referred to as identified if there is a matching peptide with greater than 95% probability.
If help/training is required in the use of MASCOT contact a member of the Proteomics Facility. We will be pleased to
provide you with assistance.
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