Perkin-Elmer Pro-Xpress
Multi-Wavelength Fluorimager

The Pro-Xpress is a multi-wavelength fluoro-imager capable of optical density and fluorescence measurements on a variety of media including 2D gels, microscope slides, tissue culture plates and exposed film. It has a maximum scanning area of 234 x 275 mm at resolutions of up to 50 um

Fluoroscence measurements can be made using combinations of up to excitation and emission wavelengths in the range 400 to 700 nm.

The sample is placed horizontally on the carrier plate (see photograph), which is automatically loaded into the instrument when a scan is requested. A cooled CCD camera scans the required area, capturing a number of FRAMES, which are combined to produce an IMAGE of the full sample.

A high resolution mosaic image is produced by illuminating the sample from:

i) below - with an ultraviolet light source for densitometry (transmission mode).

ii) above - with a high power xenon light source and interference filters for densitometry (reflection mode).

iii) above - with a high power xenon light source and interference filters for fluorescence measurements (top excitation mode).

iv) edge - with a high power xenon light source and interference filters for fluorescence measurements (translution mode).

iii) none - for chemiluminescence applications.