Tryptic Digestion Protocols have been optimised based on the following reference:

Speicher,K.D.; Kolbas,O.K; Haper,S.; Speicher,D.W.
'Systematic analysis of peptide recoveries from in-gel digestions for protein identifications in proteome studies'.
J. Biomol. Tech., 2000, 11, 74-86

In-gel Digests are usually performed using the Investigator Pro-Gest robot (see Instrumentation), using Iodoacetamide as the akylating agent.