You are here - Home ::
Mathematics of gradient separation
Two things are currently available on this site:
- A summary of the arithmetic of compartmentation
- A download of a programme to calculate compartmentation from gradient experiments
The arithmetic summary describes three main ways to calculate the distribution of some
Thingy between different compartments of a cell following a fractionation experiment
(for instance a gradient centrifugation or something). It is written by an arithmetic-aware
biologist for other biologists, so if you spot an error, please let me know. Also, if you
are a proper statistician/mathematician/numerical analyst, please don't judge me too
harshly, but let me know where I've committed too great a crime...
The software download is an old DOS application, recently resurrected. I believe it to generate the right answers, but it is not extensively tested. It takes its input as a text file of measurements, and tells you the answer on screen. Please be careful to check the answers make sense. It comes with an explanatory Word document describing how to use it, how to present the data, and how it works. I've included the full source code for anyone who is interested. It was written in Borland Turbo Pascal 6.0 (which reveals its vintage) but would translate into C easily.